Improvement of Plasmid Volumetric Yield by Addition of Glycerol and Phosphate Buffer in Escherichia coli TOP10 Batch Culture

The investigation of mRNA development has gained substantial interest, particularly in the ex vivo and in vivo therapy. mRNA is widely used for the development of gene editing-based therapies and mRNA vaccines. The aim of this study was to optimize the medium and harvest time to increase plasmid DN...

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Bibliographic Details
Main Authors: Anindyajati, Salma Aulia Afifah, Catur Riani, Marselina Irasonia Tan, Dessy Natalia, Ernawati Arifin Giri-Rachman, Anita Artarini
Format: Article
Language:English
Published: Bogor Agricultural University 2024-02-01
Series:Hayati Journal of Biosciences
Online Access:https://journal.ipb.ac.id/index.php/hayati/article/view/50976