Using mechanical homogenization to isolate microglia from mouse brain tissue to preserve transcriptomic integrity

Using mechanical homogenization to isolate microglia from mouse brain tissue to preserve transcriptomic integrity

Summary: Numerous approaches have been developed to isolate microglia from the brain, but procedures using enzymatic dissociation at 37°C can introduce drastic transcriptomic changes and confound results from gene expression assays. Here, we present an optimized protocol for microglia isolation usin...

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Bibliographic Details
Main Authors: Shawn Herron, Jean-Christophe Delpech, Charlotte Madore, Tsuneya Ikezu
Format: Article
Language:English
Published: Elsevier 2022-12-01
Series:STAR Protocols
Subjects:
Classification Description: Cell biology
Cell isolation
Flow cytometry/Mass cytometry
Cell separation/fractionation
RNAseq
Molecular biology
Online Access:http://www.sciencedirect.com/science/article/pii/S2666166722005500

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http://www.sciencedirect.com/science/article/pii/S2666166722005500

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