High-content assays for evaluating cellular and hepatic diacylglycerol acyltransferase activity

High-content assays for evaluating cellular and hepatic diacylglycerol acyltransferase activity

Acyl-CoA:diacylglycerol acyltransferase (DGAT) catalyzes the terminal step in triglyceride (TG) synthesis using diacylglycerol (DAG) and fatty acyl-CoA as substrates. In the liver, the production of VLDL permits the delivery of hydrophobic TG from the liver to peripheral tissues for energy metabolis...

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Bibliographic Details
Main Authors: Jenson Qi, Wensheng Lang, Edward Giardino, Gary W. Caldwell, Charles Smith, Lisa K. Minor, Andrew L. Darrow, Gustaaf Willemsens, Katharina DeWaepenaert, Peter Roevens, Joannes T.M. Linders, Yin Liang, Margery A. Connelly
Format: Article
Language:English
Published: Elsevier 2010-12-01
Series:Journal of Lipid Research
Subjects:
[13C18]oleic acid
triglyceride
glycerol-3-phosphate pathway
liquid chromatography/tandem mass spectrometry
high-content assay
Online Access:http://www.sciencedirect.com/science/article/pii/S0022227520313687

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http://www.sciencedirect.com/science/article/pii/S0022227520313687

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