Evaluation of Fermentative Parameters for the Production of Botryosphaeran (a(1-->3;1-->6)-β-D-glucan) and Mycelial Biomass by Botryosphaeria rhodina MAMB-05
Botryosphaeran is an exopolysaccharide (EPS) of the (1"3;1"6)-β-D-glucan type produced by the fungus Botryosphaeria rhodina MAMB-05. In attempts to enhance its extracellular production, the quantitative effects of different nitrogen sources, fermentation time, inoculum size, C/N ratio, an...
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| Format: | Article |
| Language: | English |
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Universidade Federal de Mato Grosso do Sul
2015-04-01
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| Series: | Orbital: The Electronic Journal of Chemistry |
| Subjects: | |
| Online Access: | https://periodicos.ufms.br/index.php/orbital/article/view/17899 |
| _version_ | 1797946796901662720 |
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| author | Ellen Cristine Giese Andressa F. G. Sumiya Dionísio Borsato Robert F. H. Dekker Aneli M. Barbosa |
| author_facet | Ellen Cristine Giese Andressa F. G. Sumiya Dionísio Borsato Robert F. H. Dekker Aneli M. Barbosa |
| author_sort | Ellen Cristine Giese |
| collection | DOAJ |
| description |
Botryosphaeran is an exopolysaccharide (EPS) of the (1"3;1"6)-β-D-glucan type produced by the fungus Botryosphaeria rhodina MAMB-05. In attempts to enhance its extracellular production, the quantitative effects of different nitrogen sources, fermentation time, inoculum size, C/N ratio, and initial pH were evaluated on the yields of botryosphaeran. Ammonium nitrate was selected as the best nitrogen source in examining these effects using a 2³-factorial central-composite experimental design, and analysis by the response surface method. According to a factorial design, the most important variables influencing both botryosphaeran and mycelium biomass production was inoculum concentration and the time of growth. Under these conditions, optimum botryosphaeran production occurred at 88 h of growth, 0.88 g of mycelium L-1 of nutrient mediumat C/N ratio of 30, and resulted in a 5.2 g L-1 yield of EPS in the fermentation broth. For production of the mycelium biomass, the optimum condition occurred at the same time of growth and inoculum concentration; however the best C/N ratio was 75, and resulted in a yield of 33.8 g L-1 of biomass. The results also indicated that the maximal mycelial growth is not associated to the yields of botryosphaeran production by Botryosphaeria rhodina MAMB-05.
DOI: http://dx.doi.org/10.17807/orbital.v7i1.637
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| first_indexed | 2024-04-10T21:16:37Z |
| format | Article |
| id | doaj.art-adcfd41ed3964a959f0003463a9a9a75 |
| institution | Directory Open Access Journal |
| issn | 1984-6428 |
| language | English |
| last_indexed | 2024-04-10T21:16:37Z |
| publishDate | 2015-04-01 |
| publisher | Universidade Federal de Mato Grosso do Sul |
| record_format | Article |
| series | Orbital: The Electronic Journal of Chemistry |
| spelling | doaj.art-adcfd41ed3964a959f0003463a9a9a752023-01-20T11:05:21ZengUniversidade Federal de Mato Grosso do SulOrbital: The Electronic Journal of Chemistry1984-64282015-04-0171Evaluation of Fermentative Parameters for the Production of Botryosphaeran (a(1-->3;1-->6)-β-D-glucan) and Mycelial Biomass by Botryosphaeria rhodina MAMB-05Ellen Cristine Giese0Andressa F. G. Sumiya1Dionísio Borsato2Robert F. H. Dekker3Aneli M. Barbosa41. State University of Londrina 2. Coordination for Metallurgical and Environmental Process, Center of Mineral Technology, CETEMState University of LondrinaState University of LondrinaBiorefining and Biotechnology ConsultancyState University of Londrina Botryosphaeran is an exopolysaccharide (EPS) of the (1"3;1"6)-β-D-glucan type produced by the fungus Botryosphaeria rhodina MAMB-05. In attempts to enhance its extracellular production, the quantitative effects of different nitrogen sources, fermentation time, inoculum size, C/N ratio, and initial pH were evaluated on the yields of botryosphaeran. Ammonium nitrate was selected as the best nitrogen source in examining these effects using a 2³-factorial central-composite experimental design, and analysis by the response surface method. According to a factorial design, the most important variables influencing both botryosphaeran and mycelium biomass production was inoculum concentration and the time of growth. Under these conditions, optimum botryosphaeran production occurred at 88 h of growth, 0.88 g of mycelium L-1 of nutrient mediumat C/N ratio of 30, and resulted in a 5.2 g L-1 yield of EPS in the fermentation broth. For production of the mycelium biomass, the optimum condition occurred at the same time of growth and inoculum concentration; however the best C/N ratio was 75, and resulted in a yield of 33.8 g L-1 of biomass. The results also indicated that the maximal mycelial growth is not associated to the yields of botryosphaeran production by Botryosphaeria rhodina MAMB-05. DOI: http://dx.doi.org/10.17807/orbital.v7i1.637 https://periodicos.ufms.br/index.php/orbital/article/view/17899exopolysaccharidemycelium biomassshake flask optimizationresponse surface method |
| spellingShingle | Ellen Cristine Giese Andressa F. G. Sumiya Dionísio Borsato Robert F. H. Dekker Aneli M. Barbosa Evaluation of Fermentative Parameters for the Production of Botryosphaeran (a(1-->3;1-->6)-β-D-glucan) and Mycelial Biomass by Botryosphaeria rhodina MAMB-05 Orbital: The Electronic Journal of Chemistry exopolysaccharide mycelium biomass shake flask optimization response surface method |
| title | Evaluation of Fermentative Parameters for the Production of Botryosphaeran (a(1-->3;1-->6)-β-D-glucan) and Mycelial Biomass by Botryosphaeria rhodina MAMB-05 |
| title_full | Evaluation of Fermentative Parameters for the Production of Botryosphaeran (a(1-->3;1-->6)-β-D-glucan) and Mycelial Biomass by Botryosphaeria rhodina MAMB-05 |
| title_fullStr | Evaluation of Fermentative Parameters for the Production of Botryosphaeran (a(1-->3;1-->6)-β-D-glucan) and Mycelial Biomass by Botryosphaeria rhodina MAMB-05 |
| title_full_unstemmed | Evaluation of Fermentative Parameters for the Production of Botryosphaeran (a(1-->3;1-->6)-β-D-glucan) and Mycelial Biomass by Botryosphaeria rhodina MAMB-05 |
| title_short | Evaluation of Fermentative Parameters for the Production of Botryosphaeran (a(1-->3;1-->6)-β-D-glucan) and Mycelial Biomass by Botryosphaeria rhodina MAMB-05 |
| title_sort | evaluation of fermentative parameters for the production of botryosphaeran a 1 3 1 6 β d glucan and mycelial biomass by botryosphaeria rhodina mamb 05 |
| topic | exopolysaccharide mycelium biomass shake flask optimization response surface method |
| url | https://periodicos.ufms.br/index.php/orbital/article/view/17899 |
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