Classification of 16S rRNA reads is improved using a niche-specific database constructed by near-full length sequencing.

Classification of 16S rRNA reads is improved using a niche-specific database constructed by near-full length sequencing.

Surveys of microbial populations in environmental niches of interest often utilize sequence variation in the gene encoding the ribosomal small subunit (the 16S rRNA gene). Generally, these surveys target the 16S genes using semi-degenerate primers to amplify portions of a subset of bacterial species...

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Main Authors: Phillip R Myer, Tara G McDaneld, Larry A Kuehn, Keith D Dedonder, Michael D Apley, Sarah F Capik, Brian V Lubbers, Gregory P Harhay, Dayna M Harhay, John W Keele, Madison T Henniger, Brooke A Clemmons, Timothy P L Smith
Format: Article
Language:English
Published: Public Library of Science (PLoS) 2020-01-01
Series:PLoS ONE
Online Access:https://doi.org/10.1371/journal.pone.0235498

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https://doi.org/10.1371/journal.pone.0235498

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