Immunoregulatory effect of Lactobacillus paracasei VL8 exopolysaccharide on RAW264.7 cells by NF-κB and MAPK pathways
This study explored the immunoregulatory mechanism of VL8-EPS, obtained from Viili. An RAW264.7 macrophage cell-line was used along with real-time quantitative PCR to evaluate the impact of VL8-EPS on mRNA expression of IL-1β, TNF-α, IL-6, IL-10, and TLR4. Moreover, western blot assay was conducted...
| Main Authors: | , , , , , , , |
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| Format: | Article |
| Language: | English |
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Elsevier
2022-08-01
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| Series: | Journal of Functional Foods |
| Subjects: | |
| Online Access: | http://www.sciencedirect.com/science/article/pii/S1756464622002365 |
| _version_ | 1818502303383027712 |
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| author | Jie Gao Qi Li Yuwei Liu Bing Yang Faizan Ahmed Sadiqb Xiyu Li Si Mi Yaxin Sang |
| author_facet | Jie Gao Qi Li Yuwei Liu Bing Yang Faizan Ahmed Sadiqb Xiyu Li Si Mi Yaxin Sang |
| author_sort | Jie Gao |
| collection | DOAJ |
| description | This study explored the immunoregulatory mechanism of VL8-EPS, obtained from Viili. An RAW264.7 macrophage cell-line was used along with real-time quantitative PCR to evaluate the impact of VL8-EPS on mRNA expression of IL-1β, TNF-α, IL-6, IL-10, and TLR4. Moreover, western blot assay was conducted to study the signal pathway of NF-κB and MAPK. The VL8-EPS was found to upregulate the mRNA expression of these cytokines and TLR4, while it promoted protein phosphorylation of p65, IκBα, ERK1/2, and JNK. The immunofluorescence staining and inhibitor blocking experiments proved that TLR4, NF-κB, and MAPK signal pathway were involved to secrete immune-enhancing substances. In conclusion, VL8-EPS was involved to activate downstream signal pathways of NF-κB and MAPK through TLR4 identification, which caused an increase in mRNA expression and cytokines release, ultimately exerting immune regulation. |
| first_indexed | 2024-12-10T21:08:27Z |
| format | Article |
| id | doaj.art-ae143a8a550b478db90aaf6770f87dd0 |
| institution | Directory Open Access Journal |
| issn | 1756-4646 |
| language | English |
| last_indexed | 2024-12-10T21:08:27Z |
| publishDate | 2022-08-01 |
| publisher | Elsevier |
| record_format | Article |
| series | Journal of Functional Foods |
| spelling | doaj.art-ae143a8a550b478db90aaf6770f87dd02022-12-22T01:33:33ZengElsevierJournal of Functional Foods1756-46462022-08-0195105166Immunoregulatory effect of Lactobacillus paracasei VL8 exopolysaccharide on RAW264.7 cells by NF-κB and MAPK pathwaysJie Gao0Qi Li1Yuwei Liu2Bing Yang3Faizan Ahmed Sadiqb4Xiyu Li5Si Mi6Yaxin Sang7Hebei Agriculture University, No. 2596, Lekai South Street, Baoding, Hebei 86-071000, ChinaHebei Agriculture University, No. 2596, Lekai South Street, Baoding, Hebei 86-071000, ChinaHebei Agriculture University, No. 2596, Lekai South Street, Baoding, Hebei 86-071000, ChinaHebei Agriculture University, No. 2596, Lekai South Street, Baoding, Hebei 86-071000, ChinaSchool of Food Science and Technology, Jiangnan University, Wuxi, ChinaHebei Agriculture University, No. 2596, Lekai South Street, Baoding, Hebei 86-071000, ChinaHebei Agriculture University, No. 2596, Lekai South Street, Baoding, Hebei 86-071000, ChinaHebei Agriculture University, No. 2596, Lekai South Street, Baoding, Hebei 86-071000, China; Corresponding author at: No. 2596, Lekai South Street, Lianchi District, Baoding, Hebei 86-071000, China.This study explored the immunoregulatory mechanism of VL8-EPS, obtained from Viili. An RAW264.7 macrophage cell-line was used along with real-time quantitative PCR to evaluate the impact of VL8-EPS on mRNA expression of IL-1β, TNF-α, IL-6, IL-10, and TLR4. Moreover, western blot assay was conducted to study the signal pathway of NF-κB and MAPK. The VL8-EPS was found to upregulate the mRNA expression of these cytokines and TLR4, while it promoted protein phosphorylation of p65, IκBα, ERK1/2, and JNK. The immunofluorescence staining and inhibitor blocking experiments proved that TLR4, NF-κB, and MAPK signal pathway were involved to secrete immune-enhancing substances. In conclusion, VL8-EPS was involved to activate downstream signal pathways of NF-κB and MAPK through TLR4 identification, which caused an increase in mRNA expression and cytokines release, ultimately exerting immune regulation.http://www.sciencedirect.com/science/article/pii/S1756464622002365PolysaccharideLactobacillus paracaseiRAW264.7 cellsImmunomodulatory activity |
| spellingShingle | Jie Gao Qi Li Yuwei Liu Bing Yang Faizan Ahmed Sadiqb Xiyu Li Si Mi Yaxin Sang Immunoregulatory effect of Lactobacillus paracasei VL8 exopolysaccharide on RAW264.7 cells by NF-κB and MAPK pathways Journal of Functional Foods Polysaccharide Lactobacillus paracasei RAW264.7 cells Immunomodulatory activity |
| title | Immunoregulatory effect of Lactobacillus paracasei VL8 exopolysaccharide on RAW264.7 cells by NF-κB and MAPK pathways |
| title_full | Immunoregulatory effect of Lactobacillus paracasei VL8 exopolysaccharide on RAW264.7 cells by NF-κB and MAPK pathways |
| title_fullStr | Immunoregulatory effect of Lactobacillus paracasei VL8 exopolysaccharide on RAW264.7 cells by NF-κB and MAPK pathways |
| title_full_unstemmed | Immunoregulatory effect of Lactobacillus paracasei VL8 exopolysaccharide on RAW264.7 cells by NF-κB and MAPK pathways |
| title_short | Immunoregulatory effect of Lactobacillus paracasei VL8 exopolysaccharide on RAW264.7 cells by NF-κB and MAPK pathways |
| title_sort | immunoregulatory effect of lactobacillus paracasei vl8 exopolysaccharide on raw264 7 cells by nf κb and mapk pathways |
| topic | Polysaccharide Lactobacillus paracasei RAW264.7 cells Immunomodulatory activity |
| url | http://www.sciencedirect.com/science/article/pii/S1756464622002365 |
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