Cyanidin-3-galactoside ameliorates silica-induced pulmonary fibrosis by inhibiting fibroblast differentiation via Nrf2/p38/Akt/NOX4
Aronia melanocarpa is rich in anthocyanins with many beneficial effects. Cyanidin-3-galactoside (C3G), the most abundant anthocyanin in Aronia melanocarpa was extracted and applied to silica particle-induced (SP-induced) pulmonary fibrosis mice and transforming growth factor-β-induced differentiatio...
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| Format: | Article |
| Language: | English |
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Elsevier
2022-05-01
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| Series: | Journal of Functional Foods |
| Subjects: | |
| Online Access: | http://www.sciencedirect.com/science/article/pii/S1756464622001049 |
| _version_ | 1818188450336079872 |
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| author | Chuang Ma Mingyue Lyu Chilu Deng Xia Liu Yanmin Cui Yixiao Shen Xianjun Meng Jin Zhao |
| author_facet | Chuang Ma Mingyue Lyu Chilu Deng Xia Liu Yanmin Cui Yixiao Shen Xianjun Meng Jin Zhao |
| author_sort | Chuang Ma |
| collection | DOAJ |
| description | Aronia melanocarpa is rich in anthocyanins with many beneficial effects. Cyanidin-3-galactoside (C3G), the most abundant anthocyanin in Aronia melanocarpa was extracted and applied to silica particle-induced (SP-induced) pulmonary fibrosis mice and transforming growth factor-β-induced differentiation of NIH3T3 fibroblasts cell line to investigate the anti-fibrotic properties and underlying cellular mechanisms. The administration of C3G inhibited fibroblast differentiation through antioxidant effects, thereby reducing SP-induced pulmonary fibrosis. In vivo experiments showed that C3G reduced the fibrous nodules and collagen deposition in the lungs, the level of fibroblast markers, and the secretion of pro-fibrotic factors. C3G can also maintain the balance of oxidation and antioxidant in the lungs of mice, inhibit the phosphorylation of mitogen-activated protein kinase p38 and protein kinase B, down-regulate the level of NADPH oxidase 4 and increase the level of nuclear factor (erythroid-derived 2) like 2 express. The in vitro mechanism study of NIH3T3 cells verified the results of in vivo experiments. These findings emphasized the potential use of C3G as a functional food to alleviate SP-induced pulmonary fibrosis. |
| first_indexed | 2024-12-11T23:27:07Z |
| format | Article |
| id | doaj.art-ae1be3e196ef40d7913f5db3bf6e1f1f |
| institution | Directory Open Access Journal |
| issn | 1756-4646 |
| language | English |
| last_indexed | 2024-12-11T23:27:07Z |
| publishDate | 2022-05-01 |
| publisher | Elsevier |
| record_format | Article |
| series | Journal of Functional Foods |
| spelling | doaj.art-ae1be3e196ef40d7913f5db3bf6e1f1f2022-12-22T00:46:09ZengElsevierJournal of Functional Foods1756-46462022-05-0192105034Cyanidin-3-galactoside ameliorates silica-induced pulmonary fibrosis by inhibiting fibroblast differentiation via Nrf2/p38/Akt/NOX4Chuang Ma0Mingyue Lyu1Chilu Deng2Xia Liu3Yanmin Cui4Yixiao Shen5Xianjun Meng6Jin Zhao7College of Food Science, Shenyang Agricultural University, Shenyang, Liaoning, PR ChinaCollege of Food Science, Shenyang Agricultural University, Shenyang, Liaoning, PR ChinaCollege of Food Science, Shenyang Agricultural University, Shenyang, Liaoning, PR ChinaCollege of Food Science, Shenyang Agricultural University, Shenyang, Liaoning, PR ChinaCollege of Food Science, Shenyang Agricultural University, Shenyang, Liaoning, PR ChinaCollege of Food Science, Shenyang Agricultural University, Shenyang, Liaoning, PR ChinaCollege of Food Science, Shenyang Agricultural University, Shenyang, Liaoning, PR ChinaCorresponding author.; College of Food Science, Shenyang Agricultural University, Shenyang, Liaoning, PR ChinaAronia melanocarpa is rich in anthocyanins with many beneficial effects. Cyanidin-3-galactoside (C3G), the most abundant anthocyanin in Aronia melanocarpa was extracted and applied to silica particle-induced (SP-induced) pulmonary fibrosis mice and transforming growth factor-β-induced differentiation of NIH3T3 fibroblasts cell line to investigate the anti-fibrotic properties and underlying cellular mechanisms. The administration of C3G inhibited fibroblast differentiation through antioxidant effects, thereby reducing SP-induced pulmonary fibrosis. In vivo experiments showed that C3G reduced the fibrous nodules and collagen deposition in the lungs, the level of fibroblast markers, and the secretion of pro-fibrotic factors. C3G can also maintain the balance of oxidation and antioxidant in the lungs of mice, inhibit the phosphorylation of mitogen-activated protein kinase p38 and protein kinase B, down-regulate the level of NADPH oxidase 4 and increase the level of nuclear factor (erythroid-derived 2) like 2 express. The in vitro mechanism study of NIH3T3 cells verified the results of in vivo experiments. These findings emphasized the potential use of C3G as a functional food to alleviate SP-induced pulmonary fibrosis.http://www.sciencedirect.com/science/article/pii/S1756464622001049Aronia melanocarpaCyanidin-3-galactosidePulmonary fibrosisFibroblast differentiationOxidative stress |
| spellingShingle | Chuang Ma Mingyue Lyu Chilu Deng Xia Liu Yanmin Cui Yixiao Shen Xianjun Meng Jin Zhao Cyanidin-3-galactoside ameliorates silica-induced pulmonary fibrosis by inhibiting fibroblast differentiation via Nrf2/p38/Akt/NOX4 Journal of Functional Foods Aronia melanocarpa Cyanidin-3-galactoside Pulmonary fibrosis Fibroblast differentiation Oxidative stress |
| title | Cyanidin-3-galactoside ameliorates silica-induced pulmonary fibrosis by inhibiting fibroblast differentiation via Nrf2/p38/Akt/NOX4 |
| title_full | Cyanidin-3-galactoside ameliorates silica-induced pulmonary fibrosis by inhibiting fibroblast differentiation via Nrf2/p38/Akt/NOX4 |
| title_fullStr | Cyanidin-3-galactoside ameliorates silica-induced pulmonary fibrosis by inhibiting fibroblast differentiation via Nrf2/p38/Akt/NOX4 |
| title_full_unstemmed | Cyanidin-3-galactoside ameliorates silica-induced pulmonary fibrosis by inhibiting fibroblast differentiation via Nrf2/p38/Akt/NOX4 |
| title_short | Cyanidin-3-galactoside ameliorates silica-induced pulmonary fibrosis by inhibiting fibroblast differentiation via Nrf2/p38/Akt/NOX4 |
| title_sort | cyanidin 3 galactoside ameliorates silica induced pulmonary fibrosis by inhibiting fibroblast differentiation via nrf2 p38 akt nox4 |
| topic | Aronia melanocarpa Cyanidin-3-galactoside Pulmonary fibrosis Fibroblast differentiation Oxidative stress |
| url | http://www.sciencedirect.com/science/article/pii/S1756464622001049 |
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