Reduction of lns-1 gene expression and tissue insulin levels in n5-STZ rats
Objective: The high global incidence of type 2 diabetes has challenged researchers to establish animal models that resemble the chronic stage observed in type 2 diabetes patients. One such model is induced by neonatal streptozotocin (n-STZ) administration to rat pups at 0, 2, or 5 days after birth....
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| Format: | Article |
| Language: | English |
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BMC
2013-01-01
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| Series: | Biological Research |
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| Online Access: | http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0716-97602013000300009&lng=en&tlng=en |
| _version_ | 1828766465382154240 |
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| author | Belinda Vargas Guerrero Pedro M García López Ana E González Santiago José A Domínguez Rosales Carmen M Gurrola Díaz |
| author_facet | Belinda Vargas Guerrero Pedro M García López Ana E González Santiago José A Domínguez Rosales Carmen M Gurrola Díaz |
| author_sort | Belinda Vargas Guerrero |
| collection | DOAJ |
| description | Objective: The high global incidence of type 2 diabetes has challenged researchers to establish animal models that resemble the chronic stage observed in type 2 diabetes patients. One such model is induced by neonatal streptozotocin (n-STZ) administration to rat pups at 0, 2, or 5 days after birth. In this study, we assessed lns-1 gene expression and tissue insulin levels as well as serum concentration of glucose and insulin, insulin resistance, and histological changes of the islets of Langerhans in n5-STZ rats after 20-weeks post-induction. Methods: Wistar rat pups were randomly distributed into a control group and a streptozotocin-induced group. Experimental induction involved a single intraperitoneal injection of streptozotocin (150 mg/kg) into neonates at five days after birth. Results: At 20 weeks post-induction, streptozotocin-induced rats exhibited increased serum glucose levels, reduced serum insulin levels, impaired glucose metabolism and insulin resistance compared to control rats. Histologically, streptozotocin-induced rats exhibited atrophic islets, vacuolization, and significantly fewer insulin-positive cells. lns-1 gene expression was significantly decreased in n5-STZ rats in comparison to the control group. Conclusion: Our findings support that the n5-STZ model 20 weeks post-induction represents an appropriate experimental tool to study T2D and to evaluate novel therapeutic agents and targets that involve insulin gene expression and secretion, as well as complications caused by chronic diabetes. |
| first_indexed | 2024-12-11T07:06:39Z |
| format | Article |
| id | doaj.art-ae217b397b2041d79f15f7824ac8e82d |
| institution | Directory Open Access Journal |
| issn | 0716-9760 |
| language | English |
| last_indexed | 2024-12-11T07:06:39Z |
| publishDate | 2013-01-01 |
| publisher | BMC |
| record_format | Article |
| series | Biological Research |
| spelling | doaj.art-ae217b397b2041d79f15f7824ac8e82d2022-12-22T01:16:29ZengBMCBiological Research0716-97602013-01-0146328128810.4067/S0716-97602013000300009S0716-97602013000300009Reduction of lns-1 gene expression and tissue insulin levels in n5-STZ ratsBelinda Vargas Guerrero0Pedro M García López1Ana E González Santiago2José A Domínguez Rosales3Carmen M Gurrola Díaz4Universidad de GuadalajaraUniversidad de GuadalajaraUniversidad de GuadalajaraUniversidad de GuadalajaraUniversidad de GuadalajaraObjective: The high global incidence of type 2 diabetes has challenged researchers to establish animal models that resemble the chronic stage observed in type 2 diabetes patients. One such model is induced by neonatal streptozotocin (n-STZ) administration to rat pups at 0, 2, or 5 days after birth. In this study, we assessed lns-1 gene expression and tissue insulin levels as well as serum concentration of glucose and insulin, insulin resistance, and histological changes of the islets of Langerhans in n5-STZ rats after 20-weeks post-induction. Methods: Wistar rat pups were randomly distributed into a control group and a streptozotocin-induced group. Experimental induction involved a single intraperitoneal injection of streptozotocin (150 mg/kg) into neonates at five days after birth. Results: At 20 weeks post-induction, streptozotocin-induced rats exhibited increased serum glucose levels, reduced serum insulin levels, impaired glucose metabolism and insulin resistance compared to control rats. Histologically, streptozotocin-induced rats exhibited atrophic islets, vacuolization, and significantly fewer insulin-positive cells. lns-1 gene expression was significantly decreased in n5-STZ rats in comparison to the control group. Conclusion: Our findings support that the n5-STZ model 20 weeks post-induction represents an appropriate experimental tool to study T2D and to evaluate novel therapeutic agents and targets that involve insulin gene expression and secretion, as well as complications caused by chronic diabetes.http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0716-97602013000300009&lng=en&tlng=enchronic hyperglycemiain vivo diabetes modelslns-1 genetype 2 diabetes |
| spellingShingle | Belinda Vargas Guerrero Pedro M García López Ana E González Santiago José A Domínguez Rosales Carmen M Gurrola Díaz Reduction of lns-1 gene expression and tissue insulin levels in n5-STZ rats Biological Research chronic hyperglycemia in vivo diabetes models lns-1 gene type 2 diabetes |
| title | Reduction of lns-1 gene expression and tissue insulin levels in n5-STZ rats |
| title_full | Reduction of lns-1 gene expression and tissue insulin levels in n5-STZ rats |
| title_fullStr | Reduction of lns-1 gene expression and tissue insulin levels in n5-STZ rats |
| title_full_unstemmed | Reduction of lns-1 gene expression and tissue insulin levels in n5-STZ rats |
| title_short | Reduction of lns-1 gene expression and tissue insulin levels in n5-STZ rats |
| title_sort | reduction of lns 1 gene expression and tissue insulin levels in n5 stz rats |
| topic | chronic hyperglycemia in vivo diabetes models lns-1 gene type 2 diabetes |
| url | http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0716-97602013000300009&lng=en&tlng=en |
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