Rapid prototyping of a polymer MEMS droplet dispenser by laser-assisted 3D printing
Abstract In this work, we introduce a polymer version of a previously developed silicon MEMS drop deposition tool for surface functionalization that consists of a microcantilever integrating an open fluidic channel and a reservoir. The device is fabricated by laser stereolithography, which offers th...
| Main Authors: | , , , , , , , , , |
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| Format: | Article |
| Language: | English |
| Published: |
Nature Publishing Group
2023-07-01
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| Series: | Microsystems & Nanoengineering |
| Online Access: | https://doi.org/10.1038/s41378-023-00559-3 |
| _version_ | 1797784575856869376 |
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| author | Rémi Courson Oleksii Bratash Ali Maziz Cloé Desmet Ricardo Alvarado Meza Loïc Leroy Elodie Engel Arnaud Buhot Laurent Malaquin Thierry Leïchlé |
| author_facet | Rémi Courson Oleksii Bratash Ali Maziz Cloé Desmet Ricardo Alvarado Meza Loïc Leroy Elodie Engel Arnaud Buhot Laurent Malaquin Thierry Leïchlé |
| author_sort | Rémi Courson |
| collection | DOAJ |
| description | Abstract In this work, we introduce a polymer version of a previously developed silicon MEMS drop deposition tool for surface functionalization that consists of a microcantilever integrating an open fluidic channel and a reservoir. The device is fabricated by laser stereolithography, which offers the advantages of low-cost and fast prototyping. Additionally, thanks to the ability to process multiple materials, a magnetic base is incorporated into the cantilever for convenient handling and attachment to the holder of a robotized stage used for spotting. Droplets with diameters ranging from ∼50 µm to ∼300 µm are printed upon direct contact of the cantilever tip with the surface to pattern. Liquid loading is achieved by fully immersing the cantilever into a reservoir drop, where a single load results in the deposition of more than 200 droplets. The influences of the size and shape of the cantilever tip and the reservoir on the printing outcome are studied. As a proof-of-concept of the biofunctionalization capability of this 3D printed droplet dispenser, microarrays of oligonucleotides and antibodies displaying high specificity and no cross-contamination are fabricated, and droplets are deposited at the tip of an optical fiber bundle. |
| first_indexed | 2024-03-13T00:41:51Z |
| format | Article |
| id | doaj.art-aee40f935e9b4a99b4fce64ecdf74dd6 |
| institution | Directory Open Access Journal |
| issn | 2055-7434 |
| language | English |
| last_indexed | 2024-03-13T00:41:51Z |
| publishDate | 2023-07-01 |
| publisher | Nature Publishing Group |
| record_format | Article |
| series | Microsystems & Nanoengineering |
| spelling | doaj.art-aee40f935e9b4a99b4fce64ecdf74dd62023-07-09T11:16:40ZengNature Publishing GroupMicrosystems & Nanoengineering2055-74342023-07-019111110.1038/s41378-023-00559-3Rapid prototyping of a polymer MEMS droplet dispenser by laser-assisted 3D printingRémi Courson0Oleksii Bratash1Ali Maziz2Cloé Desmet3Ricardo Alvarado Meza4Loïc Leroy5Elodie Engel6Arnaud Buhot7Laurent Malaquin8Thierry Leïchlé9LAAS-CNRS, Université de Toulouse, CNRSUniversité Grenoble Alpes, CNRS, CEA, IRIG, SyMMESLAAS-CNRS, Université de Toulouse, CNRSUniversité Grenoble Alpes, CNRS, CEA, IRIG, SyMMESUniversité Grenoble Alpes, CNRS, CEA, IRIG, SyMMESUniversité Grenoble Alpes, CNRS, CEA, IRIG, SyMMESUniversité Grenoble Alpes, CNRS, CEA, IRIG, SyMMESUniversité Grenoble Alpes, CNRS, CEA, IRIG, SyMMESLAAS-CNRS, Université de Toulouse, CNRSLAAS-CNRS, Université de Toulouse, CNRSAbstract In this work, we introduce a polymer version of a previously developed silicon MEMS drop deposition tool for surface functionalization that consists of a microcantilever integrating an open fluidic channel and a reservoir. The device is fabricated by laser stereolithography, which offers the advantages of low-cost and fast prototyping. Additionally, thanks to the ability to process multiple materials, a magnetic base is incorporated into the cantilever for convenient handling and attachment to the holder of a robotized stage used for spotting. Droplets with diameters ranging from ∼50 µm to ∼300 µm are printed upon direct contact of the cantilever tip with the surface to pattern. Liquid loading is achieved by fully immersing the cantilever into a reservoir drop, where a single load results in the deposition of more than 200 droplets. The influences of the size and shape of the cantilever tip and the reservoir on the printing outcome are studied. As a proof-of-concept of the biofunctionalization capability of this 3D printed droplet dispenser, microarrays of oligonucleotides and antibodies displaying high specificity and no cross-contamination are fabricated, and droplets are deposited at the tip of an optical fiber bundle.https://doi.org/10.1038/s41378-023-00559-3 |
| spellingShingle | Rémi Courson Oleksii Bratash Ali Maziz Cloé Desmet Ricardo Alvarado Meza Loïc Leroy Elodie Engel Arnaud Buhot Laurent Malaquin Thierry Leïchlé Rapid prototyping of a polymer MEMS droplet dispenser by laser-assisted 3D printing Microsystems & Nanoengineering |
| title | Rapid prototyping of a polymer MEMS droplet dispenser by laser-assisted 3D printing |
| title_full | Rapid prototyping of a polymer MEMS droplet dispenser by laser-assisted 3D printing |
| title_fullStr | Rapid prototyping of a polymer MEMS droplet dispenser by laser-assisted 3D printing |
| title_full_unstemmed | Rapid prototyping of a polymer MEMS droplet dispenser by laser-assisted 3D printing |
| title_short | Rapid prototyping of a polymer MEMS droplet dispenser by laser-assisted 3D printing |
| title_sort | rapid prototyping of a polymer mems droplet dispenser by laser assisted 3d printing |
| url | https://doi.org/10.1038/s41378-023-00559-3 |
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