Optimization of CRISPR/Cas9 genome editing in cotton by improved sgRNA expression

Optimization of CRISPR/Cas9 genome editing in cotton by improved sgRNA expression

Abstract Background When developing CRISPR/Cas9 systems for crops, it is crucial to invest time characterizing the genome editing efficiency of the CRISPR/Cas9 cassettes, especially if the transformation system is difficult or time-consuming. Cotton is an important crop for the production of fiber,...

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Bibliographic Details
Main Authors: Lu Long, Dan-Dan Guo, Wei Gao, Wen-Wen Yang, Li-Pan Hou, Xiao-Nan Ma, Yu-Chen Miao, Jose Ramon Botella, Chun-Peng Song
Format: Article
Language:English
Published: BMC 2018-10-01
Series:Plant Methods
Subjects:
CRISPR/Cas9
U6 promoter
Genome editing
Target mutagenesis
Transient expression
Online Access:http://link.springer.com/article/10.1186/s13007-018-0353-0

Internet

http://link.springer.com/article/10.1186/s13007-018-0353-0

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