Maximizing CRISPRi efficacy and accessibility with dual-sgRNA libraries and optimal effectors

Maximizing CRISPRi efficacy and accessibility with dual-sgRNA libraries and optimal effectors

CRISPR interference (CRISPRi) enables programmable, reversible, and titratable repression of gene expression (knockdown) in mammalian cells. Initial CRISPRi-mediated genetic screens have showcased the potential to address basic questions in cell biology, genetics, and biotechnology, but wider deploy...

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Bibliographic Details
Main Authors: Joseph M Replogle, Jessica L Bonnar, Angela N Pogson, Christina R Liem, Nolan K Maier, Yufang Ding, Baylee J Russell, Xingren Wang, Kun Leng, Alina Guna, Thomas M Norman, Ryan A Pak, Daniel M Ramos, Michael E Ward, Luke A Gilbert, Martin Kampmann, Jonathan S Weissman, Marco Jost
Format: Article
Language:English
Published: eLife Sciences Publications Ltd 2022-12-01
Series:eLife
Subjects:
CRISPR
CRISPR interference
genetic screen
functional genomics
knockdown
Online Access:https://elifesciences.org/articles/81856

Internet

https://elifesciences.org/articles/81856

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