Viral Traits and Cellular Knock-Out Genotype Affect Dependence of BVDV on Bovine CD46
The role of bovine CD46 in the host cell entry of BVDV has been established for more than a decade. By generating novel MDBK CD46 knock-out clones, we confirm previously reported data on the CD46 motives important for BVDV binding and the importance of the G479R exchange within BVDV E<sup>rns&...
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MDPI AG
2021-12-01
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| Series: | Pathogens |
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| Online Access: | https://www.mdpi.com/2076-0817/10/12/1620 |
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| author | Hann-Wei Chen Verena Huber Kati Szakmary-Braendle Kerstin Seitz Marlene Moetz Till Ruemenapf Christiane Riedel |
| author_facet | Hann-Wei Chen Verena Huber Kati Szakmary-Braendle Kerstin Seitz Marlene Moetz Till Ruemenapf Christiane Riedel |
| author_sort | Hann-Wei Chen |
| collection | DOAJ |
| description | The role of bovine CD46 in the host cell entry of BVDV has been established for more than a decade. By generating novel MDBK CD46 knock-out clones, we confirm previously reported data on the CD46 motives important for BVDV binding and the importance of the G479R exchange within BVDV E<sup>rns</sup> to gain independence of bovine CD46 during entry. The comparison of different knock-out genotypes revealed a high variability of cellular susceptibility for a BVDV encoding the G479R exchange. These data highlight the effect of clonal selection of knock-outs on virus susceptibility, which should be considered when planning knock-out experiments. |
| first_indexed | 2024-03-10T03:21:43Z |
| format | Article |
| id | doaj.art-af560a4146a041f4ae5eed2d2fef3b06 |
| institution | Directory Open Access Journal |
| issn | 2076-0817 |
| language | English |
| last_indexed | 2024-03-10T03:21:43Z |
| publishDate | 2021-12-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Pathogens |
| spelling | doaj.art-af560a4146a041f4ae5eed2d2fef3b062023-11-23T10:01:34ZengMDPI AGPathogens2076-08172021-12-011012162010.3390/pathogens10121620Viral Traits and Cellular Knock-Out Genotype Affect Dependence of BVDV on Bovine CD46Hann-Wei Chen0Verena Huber1Kati Szakmary-Braendle2Kerstin Seitz3Marlene Moetz4Till Ruemenapf5Christiane Riedel6Institute of Virology, Department of Pathobiology, University of Veterinary Medicine, 1210 Vienna, AustriaInstitute of Virology, Department of Pathobiology, University of Veterinary Medicine, 1210 Vienna, AustriaInstitute of Virology, Department of Pathobiology, University of Veterinary Medicine, 1210 Vienna, AustriaInstitute of Virology, Department of Pathobiology, University of Veterinary Medicine, 1210 Vienna, AustriaInstitute of Virology, Department of Pathobiology, University of Veterinary Medicine, 1210 Vienna, AustriaInstitute of Virology, Department of Pathobiology, University of Veterinary Medicine, 1210 Vienna, AustriaInstitute of Virology, Department of Pathobiology, University of Veterinary Medicine, 1210 Vienna, AustriaThe role of bovine CD46 in the host cell entry of BVDV has been established for more than a decade. By generating novel MDBK CD46 knock-out clones, we confirm previously reported data on the CD46 motives important for BVDV binding and the importance of the G479R exchange within BVDV E<sup>rns</sup> to gain independence of bovine CD46 during entry. The comparison of different knock-out genotypes revealed a high variability of cellular susceptibility for a BVDV encoding the G479R exchange. These data highlight the effect of clonal selection of knock-outs on virus susceptibility, which should be considered when planning knock-out experiments.https://www.mdpi.com/2076-0817/10/12/1620bovine viral diarrhea viruspestivirusbovine CD46susceptibilityCRISPR-CAS9 mediated knock-out |
| spellingShingle | Hann-Wei Chen Verena Huber Kati Szakmary-Braendle Kerstin Seitz Marlene Moetz Till Ruemenapf Christiane Riedel Viral Traits and Cellular Knock-Out Genotype Affect Dependence of BVDV on Bovine CD46 Pathogens bovine viral diarrhea virus pestivirus bovine CD46 susceptibility CRISPR-CAS9 mediated knock-out |
| title | Viral Traits and Cellular Knock-Out Genotype Affect Dependence of BVDV on Bovine CD46 |
| title_full | Viral Traits and Cellular Knock-Out Genotype Affect Dependence of BVDV on Bovine CD46 |
| title_fullStr | Viral Traits and Cellular Knock-Out Genotype Affect Dependence of BVDV on Bovine CD46 |
| title_full_unstemmed | Viral Traits and Cellular Knock-Out Genotype Affect Dependence of BVDV on Bovine CD46 |
| title_short | Viral Traits and Cellular Knock-Out Genotype Affect Dependence of BVDV on Bovine CD46 |
| title_sort | viral traits and cellular knock out genotype affect dependence of bvdv on bovine cd46 |
| topic | bovine viral diarrhea virus pestivirus bovine CD46 susceptibility CRISPR-CAS9 mediated knock-out |
| url | https://www.mdpi.com/2076-0817/10/12/1620 |
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