Multiplex Site-Directed Gene Editing Using Polyethylene Glycol-Mediated Delivery of CRISPR gRNA:Cas9 Ribonucleoprotein (RNP) Complexes to Carrot Protoplasts

Multiplex Site-Directed Gene Editing Using Polyethylene Glycol-Mediated Delivery of CRISPR gRNA:Cas9 Ribonucleoprotein (RNP) Complexes to Carrot Protoplasts

The aim of this work was to show an efficient, recombinant DNA-free, multiplex gene-editing method using gRNA:Cas9 ribonucleoprotein (RNP) complexes delivered directly to plant protoplasts. For this purpose, three RNPs were formed in the tube, their activity was confirmed by DNA cleavage in vitro, a...

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Bibliographic Details
Main Authors: Magdalena Klimek-Chodacka, Miron Gieniec, Rafal Baranski
Format: Article
Language:English
Published: MDPI AG 2021-10-01
Series:International Journal of Molecular Sciences
Subjects:
Cas9 protein
<i>Daucus carota</i>
genome editing
PEG
targeted mutagenesis
Online Access:https://www.mdpi.com/1422-0067/22/19/10740

Internet

https://www.mdpi.com/1422-0067/22/19/10740

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