Genome editing of Ralstonia eutropha using an electroporation-based CRISPR-Cas9 technique

Abstract Background Ralstonia eutropha is an important bacterium for the study of polyhydroxyalkanoates (PHAs) synthesis and CO2 fixation, which makes it a potential strain for industrial PHA production and attractive host for CO2 conversion. Although the bacterium is not recalcitrant to genetic man...

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Bibliographic Details
Main Authors: Bin Xiong, Zhongkang Li, Li Liu, Dongdong Zhao, Xueli Zhang, Changhao Bi
Format: Article
Language:English
Published: BMC 2018-06-01
Series:Biotechnology for Biofuels
Subjects:
Online Access:http://link.springer.com/article/10.1186/s13068-018-1170-4