Rapid and economical drug resistance profiling with Nanopore MinION for clinical specimens with low bacillary burden of Mycobacterium tuberculosis

Rapid and economical drug resistance profiling with Nanopore MinION for clinical specimens with low bacillary burden of Mycobacterium tuberculosis

Abstract Objective We designed and tested a Nanopore sequencing panel for direct tuberculosis drug resistance profiling. The panel targeted 10 resistance-associated loci. We assessed the feasibility of amplifying and sequencing these loci from 23 clinical specimens with low bacillary burden. Results...

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Bibliographic Details
Main Authors: Wai Sing Chan, Chun Hang Au, Yvonne Chung, Henry Chi Ming Leung, Dona N. Ho, Elaine Yue Ling Wong, Tak Wah Lam, Tsun Leung Chan, Edmond Shiu Kwan Ma, Bone Siu Fai Tang
Format: Article
Language:English
Published: BMC 2020-09-01
Series:BMC Research Notes
Subjects:
Antibiotic resistance
Illumina MiSeq
MDR-TB
Nanopore MinION
NGS
Tuberculosis
Online Access:http://link.springer.com/article/10.1186/s13104-020-05287-9

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http://link.springer.com/article/10.1186/s13104-020-05287-9

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