Protocol to encapsulate cerebral organoids with alginate hydrogel shell to induce volumetric compression

Summary: In vitro organoids, including cerebral organoids, are usually developed without mechanical compression, which may contribute to a delay in maturation. Here, we present a protocol for encapsulating cerebral organoids with a thin shell of low-concentration alginate hydrogel. We describe steps...

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Bibliographic Details
Main Authors: Zitian Wang, Xiaowei Tang, Davit Khutsishvili, Gan Sang, Edgar A. Galan, Jie Wang, Shaohua Ma
Format: Article
Language:English
Published: Elsevier 2024-06-01
Series:STAR Protocols
Subjects:
Online Access:http://www.sciencedirect.com/science/article/pii/S2666166724001175