Development of a Singleplex Real-Time Reverse Transcriptase PCR Assay for Pan-Dengue Virus Detection and Quantification

Development of a Singleplex Real-Time Reverse Transcriptase PCR Assay for Pan-Dengue Virus Detection and Quantification

Dengue virus (DENV) infection is a significant global health problem. There are no specific therapeutics or widely available vaccines. Early diagnosis is critical for patient management. Viral RNA detection by multiplex RT-PCR using multiple pairs of primers/probes allowing the simultaneous detectio...

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Bibliographic Details
Main Authors: Adisak Songjaeng, Somchai Thiemmeca, Dumrong Mairiang, Nuntaya Punyadee, Kessiri Kongmanas, Prachya Hansuealueang, Nattaya Tangthawornchaikul, Thaneeya Duangchinda, Juthathip Mongkolsapaya, Kanokwan Sriruksa, Wannee Limpitikul, Prida Malasit, Panisadee Avirutnan
Format: Article
Language:English
Published: MDPI AG 2022-06-01
Series:Viruses
Subjects:
dengue virus detection
dengue viral load quantification
one tube
singleplex real-time RT-PCR
DENV 3′-UTR detection
defective virus particles
Online Access:https://www.mdpi.com/1999-4915/14/6/1271

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https://www.mdpi.com/1999-4915/14/6/1271

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