Exogenous Melatonin Affects the Morphometric Characteristics and Glucosinolates during the Initial Growth Stages of Broccoli
Nowadays, there is a global surge in interest surrounding novel foods, particularly sprouts, microgreens, and baby leaves, attributed to their rich content of bioactive compounds, such as phenolic derivatives, glucosinolates, and vitamins. This study delves into the impact of exogenously applied mel...
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| Format: | Article |
| Language: | English |
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MDPI AG
2024-01-01
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| Series: | Agronomy |
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| Online Access: | https://www.mdpi.com/2073-4395/14/2/286 |
| _version_ | 1797299174790660096 |
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| author | Donata Arena Hajer Ben Ammar Victor Manuel Rodriguez Pablo Velasco Gresheen Garcia Riccardo Calì Ferdinando Branca |
| author_facet | Donata Arena Hajer Ben Ammar Victor Manuel Rodriguez Pablo Velasco Gresheen Garcia Riccardo Calì Ferdinando Branca |
| author_sort | Donata Arena |
| collection | DOAJ |
| description | Nowadays, there is a global surge in interest surrounding novel foods, particularly sprouts, microgreens, and baby leaves, attributed to their rich content of bioactive compounds, such as phenolic derivatives, glucosinolates, and vitamins. This study delves into the impact of exogenously applied melatonin on novel foods derived from <i>Brassica oleracea</i> L. Two distinct cultivars of broccoli (<i>Brassica oleracea</i> var. <i>italica</i> Plenck), namely Sicilian sprouting broccoli (<i>Broccolo nero</i>) and a commercial variety (Cavolo Broccolo Ramoso Calabrese), were compared across the sprouts, microgreens, and baby leaves stages, adhering to organic farming practices. Various doses of melatonin (0, 50, and 100 µM) were administered at each harvesting stage. Plantlets were collected at different growth stages and assessed for key morphometric traits, including the weight, hypocotyl length, and cotyledon dimensions during the sprouts stage. For microgreens, the number and dimensions of the true leaves were recorded, while for baby leaves, the stem length was additionally measured. The analysis of glucosinolates was carried out using a high-performance liquid chromatograph with a diode array detector (HPLC-DAD). The results revealed significant variations among the experimental factors considered. Melatonin application significantly influenced the morphometric parameters at different growth stages, exhibiting notable variations in the weight, hypocotyl length, cotyledon width, and leaf width. The GLSs profile exhibited significant variations between the different growth stages and genotypes studied. Particularly noteworthy was the tendency for the GLSs content to be higher during the sprouts stage compared to the baby leaves stage, ranging from 24.07 to 4.61 µmol g<sup>−1</sup> d.w. from sprouts to baby leaves, respectively. |
| first_indexed | 2024-03-07T22:45:55Z |
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| id | doaj.art-af90c938f5e64d8d8ad040013508add4 |
| institution | Directory Open Access Journal |
| issn | 2073-4395 |
| language | English |
| last_indexed | 2024-03-07T22:45:55Z |
| publishDate | 2024-01-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Agronomy |
| spelling | doaj.art-af90c938f5e64d8d8ad040013508add42024-02-23T15:04:04ZengMDPI AGAgronomy2073-43952024-01-0114228610.3390/agronomy14020286Exogenous Melatonin Affects the Morphometric Characteristics and Glucosinolates during the Initial Growth Stages of BroccoliDonata Arena0Hajer Ben Ammar1Victor Manuel Rodriguez2Pablo Velasco3Gresheen Garcia4Riccardo Calì5Ferdinando Branca6Department of Agriculture, Food and Environment (Di3A), University of Catania, Via Valdisavoia 5, 95123 Catania, ItalyDepartment of Agriculture, Food and Environment (Di3A), University of Catania, Via Valdisavoia 5, 95123 Catania, ItalyGroup of Genetics, Breeding and Biochemistry of Brassicas, Misión Biológica de Galicia, Spanish Council for Scientific Research (CSIC), Apartado 28, 36080 Pontevedra, SpainGroup of Genetics, Breeding and Biochemistry of Brassicas, Misión Biológica de Galicia, Spanish Council for Scientific Research (CSIC), Apartado 28, 36080 Pontevedra, SpainDepartment of Agriculture, Food and Environment (Di3A), University of Catania, Via Valdisavoia 5, 95123 Catania, ItalyDepartment of Agriculture, Food and Environment (Di3A), University of Catania, Via Valdisavoia 5, 95123 Catania, ItalyDepartment of Agriculture, Food and Environment (Di3A), University of Catania, Via Valdisavoia 5, 95123 Catania, ItalyNowadays, there is a global surge in interest surrounding novel foods, particularly sprouts, microgreens, and baby leaves, attributed to their rich content of bioactive compounds, such as phenolic derivatives, glucosinolates, and vitamins. This study delves into the impact of exogenously applied melatonin on novel foods derived from <i>Brassica oleracea</i> L. Two distinct cultivars of broccoli (<i>Brassica oleracea</i> var. <i>italica</i> Plenck), namely Sicilian sprouting broccoli (<i>Broccolo nero</i>) and a commercial variety (Cavolo Broccolo Ramoso Calabrese), were compared across the sprouts, microgreens, and baby leaves stages, adhering to organic farming practices. Various doses of melatonin (0, 50, and 100 µM) were administered at each harvesting stage. Plantlets were collected at different growth stages and assessed for key morphometric traits, including the weight, hypocotyl length, and cotyledon dimensions during the sprouts stage. For microgreens, the number and dimensions of the true leaves were recorded, while for baby leaves, the stem length was additionally measured. The analysis of glucosinolates was carried out using a high-performance liquid chromatograph with a diode array detector (HPLC-DAD). The results revealed significant variations among the experimental factors considered. Melatonin application significantly influenced the morphometric parameters at different growth stages, exhibiting notable variations in the weight, hypocotyl length, cotyledon width, and leaf width. The GLSs profile exhibited significant variations between the different growth stages and genotypes studied. Particularly noteworthy was the tendency for the GLSs content to be higher during the sprouts stage compared to the baby leaves stage, ranging from 24.07 to 4.61 µmol g<sup>−1</sup> d.w. from sprouts to baby leaves, respectively.https://www.mdpi.com/2073-4395/14/2/286<i>Brassica oleracea</i> var. <i>italica</i>landracenovel foodelicitorssecondary metaboliteHPLC analysis |
| spellingShingle | Donata Arena Hajer Ben Ammar Victor Manuel Rodriguez Pablo Velasco Gresheen Garcia Riccardo Calì Ferdinando Branca Exogenous Melatonin Affects the Morphometric Characteristics and Glucosinolates during the Initial Growth Stages of Broccoli Agronomy <i>Brassica oleracea</i> var. <i>italica</i> landrace novel food elicitors secondary metabolite HPLC analysis |
| title | Exogenous Melatonin Affects the Morphometric Characteristics and Glucosinolates during the Initial Growth Stages of Broccoli |
| title_full | Exogenous Melatonin Affects the Morphometric Characteristics and Glucosinolates during the Initial Growth Stages of Broccoli |
| title_fullStr | Exogenous Melatonin Affects the Morphometric Characteristics and Glucosinolates during the Initial Growth Stages of Broccoli |
| title_full_unstemmed | Exogenous Melatonin Affects the Morphometric Characteristics and Glucosinolates during the Initial Growth Stages of Broccoli |
| title_short | Exogenous Melatonin Affects the Morphometric Characteristics and Glucosinolates during the Initial Growth Stages of Broccoli |
| title_sort | exogenous melatonin affects the morphometric characteristics and glucosinolates during the initial growth stages of broccoli |
| topic | <i>Brassica oleracea</i> var. <i>italica</i> landrace novel food elicitors secondary metabolite HPLC analysis |
| url | https://www.mdpi.com/2073-4395/14/2/286 |
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