Optimized protocol for quantifying 5′ UTR-mediated translation initiation in S. cerevisiae using direct analysis of ribosome targeting

Optimized protocol for quantifying 5′ UTR-mediated translation initiation in S. cerevisiae using direct analysis of ribosome targeting

Summary: Direct analysis of ribosome targeting (DART) allows investigators to measure the translation initiation potential of thousands of RNAs in parallel. Here, we describe an optimized protocol for generating active translation extract from S. cerevisiae, followed by in vitro translation, purific...

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Bibliographic Details
Main Authors: Cole J.T. Lewis, Rachel O. Niederer, Ritam Neupane, Wendy V. Gilbert
Format: Article
Language:English
Published: Elsevier 2022-12-01
Series:STAR Protocols
Subjects:
Sequencing
Molecular Biology
Gene Expression
Systems biology
Online Access:http://www.sciencedirect.com/science/article/pii/S2666166722007420

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http://www.sciencedirect.com/science/article/pii/S2666166722007420

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