Genome engineering of mammalian haploid embryonic stem cells using the Cas9/RNA system

Genome engineering of mammalian haploid embryonic stem cells using the Cas9/RNA system

Haploid embryonic stem cells (ESCs) are useful for studying mammalian genes because disruption of only one allele can cause loss-of-function phenotypes. Here, we report the use of haploid ESCs and the CRISPR RNA-guided Cas9 nuclease gene-targeting system to manipulate mammalian genes. Co-transfectio...

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Bibliographic Details
Main Authors: Takuro Horii, Sumiyo Morita, Mika Kimura, Ryouhei Kobayashi, Daiki Tamura, Ryou-u Takahashi, Hironobu Kimura, Isao Suetake, Hirokazu Ohata, Koji Okamoto, Shoji Tajima, Takahiro Ochiya, Yumiko Abe, Izuho Hatada
Format: Article
Language:English
Published: PeerJ Inc. 2013-12-01
Series:PeerJ
Subjects:
CRISPR/Cas
Haploid
Embryonic stem cells
Genome engineering
Tet1
Tet2
Online Access:https://peerj.com/articles/230.pdf

Internet

https://peerj.com/articles/230.pdf

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