Generation of endogenously tagged E-cadherin cells using gene editing via non-homologous end joining

Generation of endogenously tagged E-cadherin cells using gene editing via non-homologous end joining

Summary: We provide a protocol using non-homologous end joining to integrate an oligonucleotide sequence of a fluorescence protein at the CDH1 locus encoding for the epithelial glycoprotein E-cadherin. We describe steps for implementing the CRISPR-Cas9-mediated knock-in procedure by transfecting a c...

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Bibliographic Details
Main Authors: Natalie Rimmer, Ching-Yeu Liang, Ricardo Coelho, Monica Nunez Lopez, Francis Jacob
Format: Article
Language:English
Published: Elsevier 2023-06-01
Series:STAR Protocols
Subjects:
Cell Culture
Flow Cytometry/Mass Cytometry
Cell Separation/Fractionation
Gene Expression
CRISPR
Online Access:http://www.sciencedirect.com/science/article/pii/S2666166723002721

Internet

http://www.sciencedirect.com/science/article/pii/S2666166723002721

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