Easy‐Curing and pH‐Regulated CRISPR‐Cas9 Plasmids for Gene Editing and Plasmid Curing in Lactococcus cremoris
ABSTRACT In this work, we developed a plasmid‐based CRISPR‐Cas9 strategy for editing Lactococcus cremoris, which allows easy generation of plasmid‐free strains with the desired modification. We constructed versatile shuttle vectors based on the theta‐type pAMβ1 promiscuous replicon and p15A ori, exp...
| Main Authors: | , , , |
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| Format: | Article |
| Language: | English |
| Published: |
Wiley
2024-12-01
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| Series: | Microbial Biotechnology |
| Subjects: | |
| Online Access: | https://doi.org/10.1111/1751-7915.70060 |