Polar metabolomics in human muscle biopsies using a liquid-liquid extraction and full-scan LC-MS

Polar metabolomics in human muscle biopsies using a liquid-liquid extraction and full-scan LC-MS

Summary: We describe here a user-friendly analysis protocol for semi-targeted polar metabolomics in human muscle biopsies using Zwitterionic Hydrophilic Interaction Liquid Chromatography and high-resolution full-scan mass spectrometry. Previously, this protocol has been used for Caenorhabditis elega...

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Bibliographic Details
Main Authors: Bauke V. Schomakers, Jill Hermans, Yorrick R.J. Jaspers, Gajja Salomons, Frédéric M. Vaz, Michel van Weeghel, Riekelt H. Houtkooper
Format: Article
Language:English
Published: Elsevier 2022-06-01
Series:STAR Protocols
Subjects:
Health Sciences
Mass Spectrometry
Metabolism
Metabolomics
Online Access:http://www.sciencedirect.com/science/article/pii/S2666166722001824

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http://www.sciencedirect.com/science/article/pii/S2666166722001824

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