Direct RAPD evaluation of bacteria without conventional DNA extraction
The present work reports successful DNA amplification of Pantoea agglomerans and Bacillus pumilus through Random Amplified Polymorphic DNA (RAPD). For this, template DNA was obtained without conventional DNA extraction. The procedure was as follows: cultures grown for 20 hours in 5 mL LB medium were...
| المؤلفون الرئيسيون: | , , |
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| التنسيق: | مقال |
| اللغة: | English |
| منشور في: |
Instituto de Tecnologia do Paraná (Tecpar)
2004-07-01
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| سلاسل: | Brazilian Archives of Biology and Technology |
| الموضوعات: | |
| الوصول للمادة أونلاين: | http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132004000300006 |