Expression and enzymatic activity analysis of non-structure protein 14 of SARS-CoV-2

Expression and enzymatic activity analysis of non-structure protein 14 of SARS-CoV-2

Objective To obtain the non-structure protein 14 (Nsp14) of severe acute respiratory syndrome coronavirus 2(SARS-CoV-2) with higher purity and enzymatic activity. Methods This study firstly analyzed the rare codons in the gene of nsp14 according to the codon usage bias of E. coli, followed by codon...

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Bibliographic Details
Main Author: CHEN Shuang, XU Jingwen, DING Kaiyun, LIU Jiansheng, PENG Xiaozhong, LIU Hongqi
Format: Article
Language:zho
Published: Institute of Basic Medical Sciences and Peking Union Medical College Hospital, Chinese Academy of Medical Sciences / Peking Union Medical College. 2023-01-01
Series:Jichu yixue yu linchuang
Subjects:
severe acute respiratory syndrome coronavirus 2(sars-cov-2)|non-structure protein 14(nsp14)|prokaryotic expression|protein purification|nuclease activity
Online Access:http://journal11.magtechjournal.com/Jwk_jcyxylc/fileup/1001-6325/PDF/1001-6325-2023-43-1-51.pdf

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http://journal11.magtechjournal.com/Jwk_jcyxylc/fileup/1001-6325/PDF/1001-6325-2023-43-1-51.pdf

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