APC protein immunoexpression in colorectal adenoma and adenocarcinoma
Background: activation of the Wnt pathway by mutated APC gene is considered the initial event in colorectal carcinogenesis. The identification of these mutations can improve the specific treatment of the adenocarcinoma. Objective: detect and evaluate wild-type APC protein in tissue from colorectal a...
| Main Authors: | , , , , , , , |
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| Format: | Article |
| Language: | English |
| Published: |
Thieme Revinter Publicações Ltda.
2013-07-01
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| Series: | Journal of Coloproctology |
| Online Access: | http://www.sciencedirect.com/science/article/pii/S2237936313000373 |
| _version_ | 1831656350725177344 |
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| author | Vivian Sati Oba Bourroul Guilherme Muniz Bourroul Giovanna Canato Toloi Rogério Tadeu Palma Celina Tizuko Fujiyama Oshima Thiago Simão Gomes Sílvia Saiuli Miki Ihara Jaques Waisberg |
| author_facet | Vivian Sati Oba Bourroul Guilherme Muniz Bourroul Giovanna Canato Toloi Rogério Tadeu Palma Celina Tizuko Fujiyama Oshima Thiago Simão Gomes Sílvia Saiuli Miki Ihara Jaques Waisberg |
| author_sort | Vivian Sati Oba Bourroul |
| collection | DOAJ |
| description | Background: activation of the Wnt pathway by mutated APC gene is considered the initial event in colorectal carcinogenesis. The identification of these mutations can improve the specific treatment of the adenocarcinoma. Objective: detect and evaluate wild-type APC protein in tissue from colorectal adenoma, adenocarcinoma and adjacent mucosa. Methods: 42 patients that underwent surgery for adenocarcinoma and 53 patients with resected adenomas were studied. Tissue samples from the adenocarcinoma were obtained from the tumor and from adjacent non-neoplastic mucosa located 10 cm from the proximal margin of the tumor. Adenoma tissue was obtained from representative areas. Blocks of tissue microarray (TMA) were submitted to immunohistochemistry with anti-APC, with readings of positivity and intensity of immunostaining and the score of immune expression of APC protein was obtained. Results: the APC protein immune expression score showed a significantly lower expression of APC protein in the adenoma when compared with the adenocarcinoma (p < 0.0001) and adjacent mucosa (p < 0.0001). The APC protein immune expression score in the colorectal mucosa and adjacent to the adenocarcinoma showed no significant difference (p = 0.24). Conclusions: the finding of decreased expression of APC protein in adenoma tissue may indicate that the mutated APC gene may contribute to the changes in the adenoma-carcinoma process of carcinogenesis sequence. The strong expression of protein APC in tissues from the carcinoma and adjacent mucosa suggests that in most patients in this series, the mutation of the APC gene did not participate in the oncogenesis mechanism. Resumo: Racional: a ativação da via Wnt pelo gene APC mutado é considerado evento inicial da carcinogênese colorretal. A identificação dessas mutações pode tornar o tratamento do adenocarcinoma mais específico. Objetivo: detectar e avaliar a proteína APC não mutada em tecidos de adenoma, adenocarcinoma e mucosa adjacente. Método: estudados 42 doentes operados de adenocarcinoma e 53 com adenomas ressecados. Tecidos de adenocarcinoma foram obtidas da neoplasia e da mucosa adjacente não neoplásica situadas a 10 cm da margem proximal do tumor. Tecidos do adenoma foram obtidas de área representativa. Blocos de tissue microarray (TMA) foram submetidos a imuno-histoquímica com anticorpo anti-APC. Avaliadas a positividade e intensidade da expressão e obtidos escores da imunoexpressão da proteína APC. Resultados: o escore da imunoexpressão da proteína APC no adenoma foi significantemente menor do que no adenocarcinoma (p < 0,0001) e na mucosa adjacente (p < 0,0001). O escore da imunoexpressão da proteína APC na mucosa adjacente e no adenocarcinoma não mostraram diferença significante (p = 0,24). Conclusões: a menor expressão da proteína APC no adenoma pode indicar que o gene APC mutado participa das alterações do processo adenoma-carcinoma. A forte expressão da proteína APC no CCR e na mucosa adjacente sugerem que a mutação do gene APC não participou da oncogênese. Keywords: Colorectal neoplasms Carcinoma, Adenoma APC Gene, Immunohistochemistry Biological tumor markers, Palavras-chave: Neoplasias colorretais Carcinoma, Adenoma Gene APC, Imunoistoquímica, Marcadores biológicos de tumor |
| first_indexed | 2024-12-19T17:06:32Z |
| format | Article |
| id | doaj.art-b0512c8181a64421b6a14e68ae468648 |
| institution | Directory Open Access Journal |
| issn | 2237-9363 |
| language | English |
| last_indexed | 2024-12-19T17:06:32Z |
| publishDate | 2013-07-01 |
| publisher | Thieme Revinter Publicações Ltda. |
| record_format | Article |
| series | Journal of Coloproctology |
| spelling | doaj.art-b0512c8181a64421b6a14e68ae4686482022-12-21T20:13:09ZengThieme Revinter Publicações Ltda.Journal of Coloproctology2237-93632013-07-01333118125APC protein immunoexpression in colorectal adenoma and adenocarcinomaVivian Sati Oba Bourroul0Guilherme Muniz Bourroul1Giovanna Canato Toloi2Rogério Tadeu Palma3Celina Tizuko Fujiyama Oshima4Thiago Simão Gomes5Sílvia Saiuli Miki Ihara6Jaques Waisberg7Service of Digestive System Surgery of Hospital do Servidor Público Estadual (HSPE), São Paulo, SP, Brazil; Service of Digestive System Surgery of Hospital Estadual Mário Covas, Santo André, SP, Brazil; Corresponding author.Service of Digestive System Surgery of Hospital Estadual Mário Covas, Santo André, SP, BrazilFaculdade de Medicina do ABC, São Paulo, SP, BrazilService of Digestive System Surgery of Hospital do Servidor Público Estadual (HSPE), São Paulo, SP, Brazil; Discipline of Service of Digestive System Surgery of Faculdade de Medicina do ABC, São Paulo, SP, BrazilLaboratory of Molecular Pathology of Escola Paulista de Medicina da Universidade Federal de São Paulo (EPM-UNIFESP), São Paulo, SP, BrazilLaboratory of Molecular Pathology, Departament of Pathology, EPM-UNIFESP, São Paulo, SP, BrazilLaboratory of Molecular Pathology, Departament of Pathology, EPM-UNIFESP, São Paulo, SP, BrazilGeneral and Digestive System Surgery of Faculdade de Medicina do ABC, São Paulo, SP, BrazilBackground: activation of the Wnt pathway by mutated APC gene is considered the initial event in colorectal carcinogenesis. The identification of these mutations can improve the specific treatment of the adenocarcinoma. Objective: detect and evaluate wild-type APC protein in tissue from colorectal adenoma, adenocarcinoma and adjacent mucosa. Methods: 42 patients that underwent surgery for adenocarcinoma and 53 patients with resected adenomas were studied. Tissue samples from the adenocarcinoma were obtained from the tumor and from adjacent non-neoplastic mucosa located 10 cm from the proximal margin of the tumor. Adenoma tissue was obtained from representative areas. Blocks of tissue microarray (TMA) were submitted to immunohistochemistry with anti-APC, with readings of positivity and intensity of immunostaining and the score of immune expression of APC protein was obtained. Results: the APC protein immune expression score showed a significantly lower expression of APC protein in the adenoma when compared with the adenocarcinoma (p < 0.0001) and adjacent mucosa (p < 0.0001). The APC protein immune expression score in the colorectal mucosa and adjacent to the adenocarcinoma showed no significant difference (p = 0.24). Conclusions: the finding of decreased expression of APC protein in adenoma tissue may indicate that the mutated APC gene may contribute to the changes in the adenoma-carcinoma process of carcinogenesis sequence. The strong expression of protein APC in tissues from the carcinoma and adjacent mucosa suggests that in most patients in this series, the mutation of the APC gene did not participate in the oncogenesis mechanism. Resumo: Racional: a ativação da via Wnt pelo gene APC mutado é considerado evento inicial da carcinogênese colorretal. A identificação dessas mutações pode tornar o tratamento do adenocarcinoma mais específico. Objetivo: detectar e avaliar a proteína APC não mutada em tecidos de adenoma, adenocarcinoma e mucosa adjacente. Método: estudados 42 doentes operados de adenocarcinoma e 53 com adenomas ressecados. Tecidos de adenocarcinoma foram obtidas da neoplasia e da mucosa adjacente não neoplásica situadas a 10 cm da margem proximal do tumor. Tecidos do adenoma foram obtidas de área representativa. Blocos de tissue microarray (TMA) foram submetidos a imuno-histoquímica com anticorpo anti-APC. Avaliadas a positividade e intensidade da expressão e obtidos escores da imunoexpressão da proteína APC. Resultados: o escore da imunoexpressão da proteína APC no adenoma foi significantemente menor do que no adenocarcinoma (p < 0,0001) e na mucosa adjacente (p < 0,0001). O escore da imunoexpressão da proteína APC na mucosa adjacente e no adenocarcinoma não mostraram diferença significante (p = 0,24). Conclusões: a menor expressão da proteína APC no adenoma pode indicar que o gene APC mutado participa das alterações do processo adenoma-carcinoma. A forte expressão da proteína APC no CCR e na mucosa adjacente sugerem que a mutação do gene APC não participou da oncogênese. Keywords: Colorectal neoplasms Carcinoma, Adenoma APC Gene, Immunohistochemistry Biological tumor markers, Palavras-chave: Neoplasias colorretais Carcinoma, Adenoma Gene APC, Imunoistoquímica, Marcadores biológicos de tumorhttp://www.sciencedirect.com/science/article/pii/S2237936313000373 |
| spellingShingle | Vivian Sati Oba Bourroul Guilherme Muniz Bourroul Giovanna Canato Toloi Rogério Tadeu Palma Celina Tizuko Fujiyama Oshima Thiago Simão Gomes Sílvia Saiuli Miki Ihara Jaques Waisberg APC protein immunoexpression in colorectal adenoma and adenocarcinoma Journal of Coloproctology |
| title | APC protein immunoexpression in colorectal adenoma and adenocarcinoma |
| title_full | APC protein immunoexpression in colorectal adenoma and adenocarcinoma |
| title_fullStr | APC protein immunoexpression in colorectal adenoma and adenocarcinoma |
| title_full_unstemmed | APC protein immunoexpression in colorectal adenoma and adenocarcinoma |
| title_short | APC protein immunoexpression in colorectal adenoma and adenocarcinoma |
| title_sort | apc protein immunoexpression in colorectal adenoma and adenocarcinoma |
| url | http://www.sciencedirect.com/science/article/pii/S2237936313000373 |
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