Efficient generation of locus-specific human CAR-T cells with CRISPR/cCas12a

Efficient generation of locus-specific human CAR-T cells with CRISPR/cCas12a

Summary: We recently developed a system to create human chimeric antigen receptor (CAR)-T cells using conjugated Cas12a (cCas12a) in which Cas12a is covalently linked to its CRISPR RNA (crRNA). This protocol describes site-specific modification of Cas12a and the preparation of Cas12a-crRNA complex u...

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Bibliographic Details
Main Authors: Xinyu Ling, Liying Chang, Heqi Chen, Tao Liu
Format: Article
Language:English
Published: Elsevier 2022-06-01
Series:STAR Protocols
Subjects:
Cell Biology
Cell culture
Cell isolation
Flow Cytometry/Mass Cytometry
Immunology
Molecular Biology
Online Access:http://www.sciencedirect.com/science/article/pii/S2666166722002015

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http://www.sciencedirect.com/science/article/pii/S2666166722002015

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