An Organic Solvent-Free Method for the Extraction of Ellagic Acid Compounds from Raspberry Wine Pomace with Assistance of Sodium Bicarbonate
Industrial processing of raspberry juice and wine generates considerable byproducts of raspberry pomace. Ellagic acids/ellagitannins, being characterized by their antioxidant and antiproliferation properties, constitute the majority of polyphenolics in the pomace and are valuable for recovery. In th...
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| Format: | Article |
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MDPI AG
2022-03-01
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| Series: | Molecules |
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| Online Access: | https://www.mdpi.com/1420-3049/27/7/2145 |
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| author | Ning Jin Shouyu Zhang Shibo Sun Minghuo Wu Xiaojing Yang Jianqiang Xu Kun Ma Shui Guan Weiping Xu |
| author_facet | Ning Jin Shouyu Zhang Shibo Sun Minghuo Wu Xiaojing Yang Jianqiang Xu Kun Ma Shui Guan Weiping Xu |
| author_sort | Ning Jin |
| collection | DOAJ |
| description | Industrial processing of raspberry juice and wine generates considerable byproducts of raspberry pomace. Ellagic acids/ellagitannins, being characterized by their antioxidant and antiproliferation properties, constitute the majority of polyphenolics in the pomace and are valuable for recovery. In the present study, we developed a novel procedure with sodium bicarbonate assisted extraction (SBAE) to recover ellagic acid from raspberry wine pomace. Key parameters in the procedure, i.e., sodium bicarbonate concentration, temperature, time and solid/liquid (S/L) ratio, were investigated by single factor analysis and optimized subsequently by Response Surface Methodology (RSM). Optimal parameters for the SBAE method here were found to be 1.2% (<i>w</i>/<i>v</i>) NaHCO<sub>3</sub>, 1:93 (<i>w</i>/<i>v</i>) S/L ratio, 22 min and 100 °C. Under these conditions, the ellagic acid yield was 6.30 ± 0.92 mg/g pomace with an antioxidant activity of 79.0 ± 0.96 μmol Trolox eq/g pomace (DPPH assay), which are 2.37 and 1.32 times the values obtained by extraction with methanol–acetone–water solvent, respectively. The considerable improvement in ellagic acid extraction efficiency could be highly attributed to the reactions of lipid saponification and ellagitannin hydrolysis resulted from sodium bicarbonates. The present study has established an organic solvent-free method for the extraction of ellagic acid from raspberry wine pomace, which is feasible and practical in nutraceutical applications. |
| first_indexed | 2024-03-09T11:37:26Z |
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| last_indexed | 2024-03-09T11:37:26Z |
| publishDate | 2022-03-01 |
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| series | Molecules |
| spelling | doaj.art-b0b3a56225a64214b919a578a4bd55532023-11-30T23:40:11ZengMDPI AGMolecules1420-30492022-03-01277214510.3390/molecules27072145An Organic Solvent-Free Method for the Extraction of Ellagic Acid Compounds from Raspberry Wine Pomace with Assistance of Sodium BicarbonateNing Jin0Shouyu Zhang1Shibo Sun2Minghuo Wu3Xiaojing Yang4Jianqiang Xu5Kun Ma6Shui Guan7Weiping Xu8School of Ocean Science and Technology & Panjin Institute of Industrial Technology, Dalian University of Technology, Panjin 124221, ChinaSchool of Ocean Science and Technology & Panjin Institute of Industrial Technology, Dalian University of Technology, Panjin 124221, ChinaSchool of Life and Pharmaceutical Sciences, Dalian University of Technology, Panjin 124221, ChinaSchool of Ocean Science and Technology & Panjin Institute of Industrial Technology, Dalian University of Technology, Panjin 124221, ChinaSchool of Ocean Science and Technology & Panjin Institute of Industrial Technology, Dalian University of Technology, Panjin 124221, ChinaSchool of Ocean Science and Technology & Panjin Institute of Industrial Technology, Dalian University of Technology, Panjin 124221, ChinaSchool of Life and Pharmaceutical Sciences, Dalian University of Technology, Panjin 124221, ChinaState Key Laboratory of Fine Chemicals, Dalian R&D Center for Stem Cell and Tissue Engineering, School of Chemical Engineering, Dalian University of Technology, Dalian 116023, ChinaSchool of Ocean Science and Technology & Panjin Institute of Industrial Technology, Dalian University of Technology, Panjin 124221, ChinaIndustrial processing of raspberry juice and wine generates considerable byproducts of raspberry pomace. Ellagic acids/ellagitannins, being characterized by their antioxidant and antiproliferation properties, constitute the majority of polyphenolics in the pomace and are valuable for recovery. In the present study, we developed a novel procedure with sodium bicarbonate assisted extraction (SBAE) to recover ellagic acid from raspberry wine pomace. Key parameters in the procedure, i.e., sodium bicarbonate concentration, temperature, time and solid/liquid (S/L) ratio, were investigated by single factor analysis and optimized subsequently by Response Surface Methodology (RSM). Optimal parameters for the SBAE method here were found to be 1.2% (<i>w</i>/<i>v</i>) NaHCO<sub>3</sub>, 1:93 (<i>w</i>/<i>v</i>) S/L ratio, 22 min and 100 °C. Under these conditions, the ellagic acid yield was 6.30 ± 0.92 mg/g pomace with an antioxidant activity of 79.0 ± 0.96 μmol Trolox eq/g pomace (DPPH assay), which are 2.37 and 1.32 times the values obtained by extraction with methanol–acetone–water solvent, respectively. The considerable improvement in ellagic acid extraction efficiency could be highly attributed to the reactions of lipid saponification and ellagitannin hydrolysis resulted from sodium bicarbonates. The present study has established an organic solvent-free method for the extraction of ellagic acid from raspberry wine pomace, which is feasible and practical in nutraceutical applications.https://www.mdpi.com/1420-3049/27/7/2145ellagic acidextractionraspberrywine pomacesodium bicarbonateresponse surface methodology |
| spellingShingle | Ning Jin Shouyu Zhang Shibo Sun Minghuo Wu Xiaojing Yang Jianqiang Xu Kun Ma Shui Guan Weiping Xu An Organic Solvent-Free Method for the Extraction of Ellagic Acid Compounds from Raspberry Wine Pomace with Assistance of Sodium Bicarbonate Molecules ellagic acid extraction raspberry wine pomace sodium bicarbonate response surface methodology |
| title | An Organic Solvent-Free Method for the Extraction of Ellagic Acid Compounds from Raspberry Wine Pomace with Assistance of Sodium Bicarbonate |
| title_full | An Organic Solvent-Free Method for the Extraction of Ellagic Acid Compounds from Raspberry Wine Pomace with Assistance of Sodium Bicarbonate |
| title_fullStr | An Organic Solvent-Free Method for the Extraction of Ellagic Acid Compounds from Raspberry Wine Pomace with Assistance of Sodium Bicarbonate |
| title_full_unstemmed | An Organic Solvent-Free Method for the Extraction of Ellagic Acid Compounds from Raspberry Wine Pomace with Assistance of Sodium Bicarbonate |
| title_short | An Organic Solvent-Free Method for the Extraction of Ellagic Acid Compounds from Raspberry Wine Pomace with Assistance of Sodium Bicarbonate |
| title_sort | organic solvent free method for the extraction of ellagic acid compounds from raspberry wine pomace with assistance of sodium bicarbonate |
| topic | ellagic acid extraction raspberry wine pomace sodium bicarbonate response surface methodology |
| url | https://www.mdpi.com/1420-3049/27/7/2145 |
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