Comparing Efficiency of Lysis Buffer Solutions and Sample Preparation Methods for Liquid Chromatography–Mass Spectrometry Analysis of Human Cells and Plasma

Comparing Efficiency of Lysis Buffer Solutions and Sample Preparation Methods for Liquid Chromatography–Mass Spectrometry Analysis of Human Cells and Plasma

The use of a proper sample processing methodology for maximum proteome coverage and high-quality quantitative data is an important choice to make before initiating a liquid chromatography–mass spectrometry (LC–MS)-based proteomics study. Popular sample processing workflows for proteomics involve in-...

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Bibliographic Details
Main Authors: Lasse Neset, Gracious Takayidza, Frode S. Berven, Maria Hernandez-Valladares
Format: Article
Language:English
Published: MDPI AG 2022-05-01
Series:Molecules
Subjects:
sodium dodecyl sulfate
guanidinium hydrochloride
liquid chromatography–mass spectrometry
proteomics
single-pot
solid-phase-enhanced sample preparation
Online Access:https://www.mdpi.com/1420-3049/27/11/3390

Internet

https://www.mdpi.com/1420-3049/27/11/3390

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