Multiplex Bioanalytical Methods for Comprehensive Characterization and Quantification of the Unique Complementarity-Determining-Region Deamidation of MEDI7247, an Anti-ASCT2 Pyrrolobenzodiazepine Antibody–Drug Conjugate

Multiplex Bioanalytical Methods for Comprehensive Characterization and Quantification of the Unique Complementarity-Determining-Region Deamidation of MEDI7247, an Anti-ASCT2 Pyrrolobenzodiazepine Antibody–Drug Conjugate

Deamidation, a common post-translational modification, may impact multiple physiochemical properties of a therapeutic protein. MEDI7247, a pyrrolobenzodiazepine (PBD) antibody–drug conjugate (ADC), contains a unique deamidation site, N102, located within the complementarity-determining region (CDR),...

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Bibliographic Details
Main Authors: Yue Huang, Jiaqi Yuan, Ruipeng Mu, Robert J. Kubiak, Kathryn Ball, Mingyan Cao, G. Patrick Hussmann, Niluka de Mel, Dengfeng Liu, Lorin K. Roskos, Meina Liang, Anton I. Rosenbaum
Format: Article
Language:English
Published: MDPI AG 2023-10-01
Series:Antibodies
Subjects:
deamidation
hybrid liquid chromatography–mass spectrometry (LC-MS) assay
post-translational modification (PTM)
biotransformation
antibody–drug conjugate (ADC)
Online Access:https://www.mdpi.com/2073-4468/12/4/66

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https://www.mdpi.com/2073-4468/12/4/66

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