Protocol for embedded 3D printing of heart tissues using thiol-norbornene collagen

Protocol for embedded 3D printing of heart tissues using thiol-norbornene collagen

Summary: Here, we present a protocol for 3D printing heart tissues using thiol-norbornene photoclick collagen (NorCol). We describe steps for synthesizing NorCol, preparing bioink and the support bath, and cell-laden printing. We then detail procedures for the loading of C2C12 cells into NorCol, ens...

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Bibliographic Details
Main Authors: Zili Gao, Shenglong Ding, Tingting Fan, Wenhui Huang, Xiyuan Zhao, Xin Liu, Wenli Liu, Mingzhu Zhang, Qi Gu
Format: Article
Language:English
Published: Elsevier 2024-06-01
Series:STAR Protocols
Subjects:
Cell Biology
Cell culture
Tissue Engineering
Biotechnology and bioengineering
Material sciences
Online Access:http://www.sciencedirect.com/science/article/pii/S266616672400159X

Internet

http://www.sciencedirect.com/science/article/pii/S266616672400159X

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