Protocol for embedded 3D printing of heart tissues using thiol-norbornene collagen

Summary: Here, we present a protocol for 3D printing heart tissues using thiol-norbornene photoclick collagen (NorCol). We describe steps for synthesizing NorCol, preparing bioink and the support bath, and cell-laden printing. We then detail procedures for the loading of C2C12 cells into NorCol, ens...

Full description

Bibliographic Details
Main Authors: Zili Gao, Shenglong Ding, Tingting Fan, Wenhui Huang, Xiyuan Zhao, Xin Liu, Wenli Liu, Mingzhu Zhang, Qi Gu
Format: Article
Language:English
Published: Elsevier 2024-06-01
Series:STAR Protocols
Subjects:
Online Access:http://www.sciencedirect.com/science/article/pii/S266616672400159X
Description
Summary:Summary: Here, we present a protocol for 3D printing heart tissues using thiol-norbornene photoclick collagen (NorCol). We describe steps for synthesizing NorCol, preparing bioink and the support bath, and cell-laden printing. We then detail procedures for the loading of C2C12 cells into NorCol, ensuring structural integrity and cell viability after printing. This protocol is adaptable to various cell lines and allows for the printing of diverse complex structures, which can be used in drug screening and disease modeling. : Publisher’s note: Undertaking any experimental protocol requires adherence to local institutional guidelines for laboratory safety and ethics.
ISSN:2666-1667