Protocol for embedded 3D printing of heart tissues using thiol-norbornene collagen
Summary: Here, we present a protocol for 3D printing heart tissues using thiol-norbornene photoclick collagen (NorCol). We describe steps for synthesizing NorCol, preparing bioink and the support bath, and cell-laden printing. We then detail procedures for the loading of C2C12 cells into NorCol, ens...
| Main Authors: | , , , , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
Elsevier
2024-06-01
|
| Series: | STAR Protocols |
| Subjects: | |
| Online Access: | http://www.sciencedirect.com/science/article/pii/S266616672400159X |
| _version_ | 1797224148451196928 |
|---|---|
| author | Zili Gao Shenglong Ding Tingting Fan Wenhui Huang Xiyuan Zhao Xin Liu Wenli Liu Mingzhu Zhang Qi Gu |
| author_facet | Zili Gao Shenglong Ding Tingting Fan Wenhui Huang Xiyuan Zhao Xin Liu Wenli Liu Mingzhu Zhang Qi Gu |
| author_sort | Zili Gao |
| collection | DOAJ |
| description | Summary: Here, we present a protocol for 3D printing heart tissues using thiol-norbornene photoclick collagen (NorCol). We describe steps for synthesizing NorCol, preparing bioink and the support bath, and cell-laden printing. We then detail procedures for the loading of C2C12 cells into NorCol, ensuring structural integrity and cell viability after printing. This protocol is adaptable to various cell lines and allows for the printing of diverse complex structures, which can be used in drug screening and disease modeling. : Publisher’s note: Undertaking any experimental protocol requires adherence to local institutional guidelines for laboratory safety and ethics. |
| first_indexed | 2024-04-24T13:48:31Z |
| format | Article |
| id | doaj.art-b0d39fb1e99f4c1fb085d1030076f3f4 |
| institution | Directory Open Access Journal |
| issn | 2666-1667 |
| language | English |
| last_indexed | 2024-04-24T13:48:31Z |
| publishDate | 2024-06-01 |
| publisher | Elsevier |
| record_format | Article |
| series | STAR Protocols |
| spelling | doaj.art-b0d39fb1e99f4c1fb085d1030076f3f42024-04-04T05:07:20ZengElsevierSTAR Protocols2666-16672024-06-0152102994Protocol for embedded 3D printing of heart tissues using thiol-norbornene collagenZili Gao0Shenglong Ding1Tingting Fan2Wenhui Huang3Xiyuan Zhao4Xin Liu5Wenli Liu6Mingzhu Zhang7Qi Gu8Laboratory of Organ Regeneration and Reconstruction, Institute of Zoology, Chinese Academy of Sciences, Beijing 100101, P.R. China; Beijing Institute for Stem Cell and Regenerative Medicine, Beijing 100101, P.R. ChinaDepartment of Foot and Ankle Surgery, Beijing Tongren Hospital, Capital Medical University, Beijing 100730, P.R. ChinaLaboratory of Organ Regeneration and Reconstruction, Institute of Zoology, Chinese Academy of Sciences, Beijing 100101, P.R. China; Beijing Institute for Stem Cell and Regenerative Medicine, Beijing 100101, P.R. ChinaLaboratory of Organ Regeneration and Reconstruction, Institute of Zoology, Chinese Academy of Sciences, Beijing 100101, P.R. China; Beijing Institute for Stem Cell and Regenerative Medicine, Beijing 100101, P.R. ChinaLaboratory of Organ Regeneration and Reconstruction, Institute of Zoology, Chinese Academy of Sciences, Beijing 100101, P.R. China; Beijing Institute for Stem Cell and Regenerative Medicine, Beijing 100101, P.R. ChinaLaboratory of Organ Regeneration and Reconstruction, Institute of Zoology, Chinese Academy of Sciences, Beijing 100101, P.R. China; Beijing Institute for Stem Cell and Regenerative Medicine, Beijing 100101, P.R. China; Key Laboratory of Organ Regeneration and Transplantation of the Ministry of Education, Jilin University, Changchun 130061, P.R. ChinaLaboratory of Organ Regeneration and Reconstruction, Institute of Zoology, Chinese Academy of Sciences, Beijing 100101, P.R. China; Beijing Institute for Stem Cell and Regenerative Medicine, Beijing 100101, P.R. ChinaDepartment of Foot and Ankle Surgery, Beijing Tongren Hospital, Capital Medical University, Beijing 100730, P.R. China; Corresponding authorLaboratory of Organ Regeneration and Reconstruction, Institute of Zoology, Chinese Academy of Sciences, Beijing 100101, P.R. China; Beijing Institute for Stem Cell and Regenerative Medicine, Beijing 100101, P.R. China; Corresponding authorSummary: Here, we present a protocol for 3D printing heart tissues using thiol-norbornene photoclick collagen (NorCol). We describe steps for synthesizing NorCol, preparing bioink and the support bath, and cell-laden printing. We then detail procedures for the loading of C2C12 cells into NorCol, ensuring structural integrity and cell viability after printing. This protocol is adaptable to various cell lines and allows for the printing of diverse complex structures, which can be used in drug screening and disease modeling. : Publisher’s note: Undertaking any experimental protocol requires adherence to local institutional guidelines for laboratory safety and ethics.http://www.sciencedirect.com/science/article/pii/S266616672400159XCell BiologyCell cultureTissue EngineeringBiotechnology and bioengineeringMaterial sciences |
| spellingShingle | Zili Gao Shenglong Ding Tingting Fan Wenhui Huang Xiyuan Zhao Xin Liu Wenli Liu Mingzhu Zhang Qi Gu Protocol for embedded 3D printing of heart tissues using thiol-norbornene collagen STAR Protocols Cell Biology Cell culture Tissue Engineering Biotechnology and bioengineering Material sciences |
| title | Protocol for embedded 3D printing of heart tissues using thiol-norbornene collagen |
| title_full | Protocol for embedded 3D printing of heart tissues using thiol-norbornene collagen |
| title_fullStr | Protocol for embedded 3D printing of heart tissues using thiol-norbornene collagen |
| title_full_unstemmed | Protocol for embedded 3D printing of heart tissues using thiol-norbornene collagen |
| title_short | Protocol for embedded 3D printing of heart tissues using thiol-norbornene collagen |
| title_sort | protocol for embedded 3d printing of heart tissues using thiol norbornene collagen |
| topic | Cell Biology Cell culture Tissue Engineering Biotechnology and bioengineering Material sciences |
| url | http://www.sciencedirect.com/science/article/pii/S266616672400159X |
| work_keys_str_mv | AT ziligao protocolforembedded3dprintingofhearttissuesusingthiolnorbornenecollagen AT shenglongding protocolforembedded3dprintingofhearttissuesusingthiolnorbornenecollagen AT tingtingfan protocolforembedded3dprintingofhearttissuesusingthiolnorbornenecollagen AT wenhuihuang protocolforembedded3dprintingofhearttissuesusingthiolnorbornenecollagen AT xiyuanzhao protocolforembedded3dprintingofhearttissuesusingthiolnorbornenecollagen AT xinliu protocolforembedded3dprintingofhearttissuesusingthiolnorbornenecollagen AT wenliliu protocolforembedded3dprintingofhearttissuesusingthiolnorbornenecollagen AT mingzhuzhang protocolforembedded3dprintingofhearttissuesusingthiolnorbornenecollagen AT qigu protocolforembedded3dprintingofhearttissuesusingthiolnorbornenecollagen |