Successful isolation of viable stem cells from cryopreserved microfragmented human adipose tissue from patients with knee osteoarthritis – a comparative study of isolation by tissue explant culture and enzymatic digestion

Successful isolation of viable stem cells from cryopreserved microfragmented human adipose tissue from patients with knee osteoarthritis – a comparative study of isolation by tissue explant culture and enzymatic digestion

Abstract Purpose To investigate if viable stem cells could be isolated and expanded from cryopreserved microfragmented adipose tissue (AT) harvested from patients with knee osteoarthritis. Methods Microfragmented abdominal AT from knee osteoarthritis patients was cryopreserved at ‐80 °C in cryoprote...

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Bibliographic Details
Main Authors: Jasmin Bagge, Per Hölmich, Freja Aabæk Hammer, Jan O. Nehlin, Kilian Vomstein, Lars Blønd, Lisbet Rosenkrantz Hölmich, Kristoffer Weisskirchner Barfod
Format: Article
Language:English
Published: Wiley 2023-01-01
Series:Journal of Experimental Orthopaedics
Subjects:
Stem cells
Cryopreservation
Biobanking
Adipose tissue
Microfragmentation
Enzymatic digestion
Online Access:https://doi.org/10.1186/s40634-023-00596-x

Internet

https://doi.org/10.1186/s40634-023-00596-x

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