Multiplexing fluorogenic esterase-based viability assay with luciferase assays

Luciferase-based reporter assays are one of the most common cell-based screening formats for drug discovery, and simultaneous evaluation of the cytotoxic effect of test compounds is of great value in reducing false-positives. Here we share a multiplex assay protocol that allows sequential measuremen...

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Bibliographic Details
Main Authors: Kenji Ohgane, Hiromasa Yoshioka, Yuichi Hashimoto
Format: Article
Language:English
Published: Elsevier 2019-01-01
Series:MethodsX
Online Access:http://www.sciencedirect.com/science/article/pii/S2215016119302328