Multiplexing fluorogenic esterase-based viability assay with luciferase assays
Luciferase-based reporter assays are one of the most common cell-based screening formats for drug discovery, and simultaneous evaluation of the cytotoxic effect of test compounds is of great value in reducing false-positives. Here we share a multiplex assay protocol that allows sequential measuremen...
Main Authors: | Kenji Ohgane, Hiromasa Yoshioka, Yuichi Hashimoto |
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Format: | Article |
Language: | English |
Published: |
Elsevier
2019-01-01
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Series: | MethodsX |
Online Access: | http://www.sciencedirect.com/science/article/pii/S2215016119302328 |
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