Microfluidic Chip with Two-Stage Isothermal Amplification Method for Highly Sensitive Parallel Detection of SARS-CoV-2 and Measles Virus

Microfluidic Chip with Two-Stage Isothermal Amplification Method for Highly Sensitive Parallel Detection of SARS-CoV-2 and Measles Virus

A two-stage isothermal amplification method, which consists of a first-stage basic recombinase polymerase amplification (RPA) and a second-stage fluorescence loop-mediated isothermal amplification (LAMP), as well as a microfluidic-chip-based portable system, were developed in this study; these enabl...

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Bibliographic Details
Main Authors: Qin Huang, Xiaohui Shan, Ranran Cao, Xiangyu Jin, Xue Lin, Qiurong He, Yulei Zhu, Rongxin Fu, Wenli Du, Wenqi Lv, Ying Xia, Guoliang Huang
Format: Article
Language:English
Published: MDPI AG 2021-12-01
Series:Micromachines
Subjects:
two-stage isothermal amplification
microfluidic chip
parallel molecular diagnostic
SARS-CoV-2
measles virus
Online Access:https://www.mdpi.com/2072-666X/12/12/1582

Internet

https://www.mdpi.com/2072-666X/12/12/1582

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