Biophysical Screening Pipeline for Cryo-EM Grid Preparation of Membrane Proteins

Biophysical Screening Pipeline for Cryo-EM Grid Preparation of Membrane Proteins

Successful sample preparation is the foundation to any structural biology technique. Membrane proteins are of particular interest as these are important targets for drug design, but also notoriously difficult to work with. For electron cryo-microscopy (cryo-EM), the biophysical characterization of s...

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Bibliographic Details
Main Authors: Stephan Niebling, Katharina Veith, Benjamin Vollmer, Javier Lizarrondo, Osvaldo Burastero, Janina Schiller, Angelica Struve García, Philipp Lewe, Carolin Seuring, Susanne Witt, María García-Alai
Format: Article
Language:English
Published: Frontiers Media S.A. 2022-06-01
Series:Frontiers in Molecular Biosciences
Subjects:
mass photometry
differential scanning fluorimetry (DSF)
membrane proteins
dynamic light scattering
sample preparation
electron cryo-microscopy (cryo-EM)
Online Access:https://www.frontiersin.org/articles/10.3389/fmolb.2022.882288/full

Internet

https://www.frontiersin.org/articles/10.3389/fmolb.2022.882288/full

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