Suspension TRAPping Filter (sTRAP) Sample Preparation for Quantitative Proteomics in the Low µg Input Range Using a Plasmid DNA Micro-Spin Column: Analysis of the Hippocampus from the 5xFAD Alzheimer’s Disease Mouse Model

Suspension TRAPping Filter (sTRAP) Sample Preparation for Quantitative Proteomics in the Low µg Input Range Using a Plasmid DNA Micro-Spin Column: Analysis of the Hippocampus from the 5xFAD Alzheimer’s Disease Mouse Model

Suspension TRAPping filter (sTRAP) is an attractive sample preparation method for proteomics studies. The sTRAP protocol uses 5% SDS that maximizes protein solubilization. Proteins are trapped on a borosilicate glass membrane filter, where SDS is subsequently removed from the filter. After trypsin d...

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Bibliographic Details
Main Authors: Evangelia Thanou, Frank Koopmans, Débora Pita-Illobre, Remco V. Klaassen, Berna Özer, Ioannis Charalampopoulos, August B. Smit, Ka Wan Li
Format: Article
Language:English
Published: MDPI AG 2023-04-01
Series:Cells
Subjects:
sTRAP
micro-spin column
Alzheimer’s disease
5xFAD mouse model
proteomics
Online Access:https://www.mdpi.com/2073-4409/12/9/1242

Internet

https://www.mdpi.com/2073-4409/12/9/1242

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