<i>Dictyostelium</i> Cell Fixation: Two Simple Tricks

We share two simple modifications to enhance the fixation and imaging of relatively small, motile, and rounded model cells. These include cell centrifugation and the addition of trace amounts of glutaraldehyde to existing fixation methods. Though they need to be carefully considered in each context,...

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Bibliographic Details
Main Authors: Michael Koonce, Irina Tikhonenko, Ralph Gräf
Format: Article
Language:English
Published: MDPI AG 2020-07-01
Series:Methods and Protocols
Subjects:
Online Access:https://www.mdpi.com/2409-9279/3/3/47