Evaluation of long-chain fatty acid respiration in neonatal mouse cardiomyocytes using SeaHorse instrument

Evaluation of long-chain fatty acid respiration in neonatal mouse cardiomyocytes using SeaHorse instrument

Summary: Metabolic switches play a critical role in the pathophysiology of cardiac diseases, including heart failure. Here, we describe an assay for long-chain fatty acid oxidation in neonatal mouse cardiomyocytes by using a SeaHorse Flux Analyzer (Agilent). This protocol is a simplified but robust...

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Bibliographic Details
Main Authors: Aude Angelini, Xinchun Pi, Liang Xie
Format: Article
Language:English
Published: Elsevier 2022-06-01
Series:STAR Protocols
Subjects:
Cell Biology
Cell culture
Cell isolation
Metabolism
Protein Biochemistry
Online Access:http://www.sciencedirect.com/science/article/pii/S2666166722002726

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http://www.sciencedirect.com/science/article/pii/S2666166722002726

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