Preparation of single cell suspensions enriched in mouse brain vascular cells for single-cell RNA sequencing

Preparation of single cell suspensions enriched in mouse brain vascular cells for single-cell RNA sequencing

Summary: Cerebral blood vessels supply oxygen and nutrients, remove metabolic waste, and play a critical role in maintaining brain homeostasis. Cerebrovasculature is composed of heterogeneous populations of brain vascular cells (BVCs). A major challenge in effective cerebrovascular transcriptional p...

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Bibliographic Details
Main Authors: Akari Yamazaki, Francis Shue, Yu Yamazaki, Yuka A. Martens, Guojun Bu, Chia-Chen Liu
Format: Article
Language:English
Published: Elsevier 2021-09-01
Series:STAR Protocols
Subjects:
Cell isolation
Neuroscience
RNAseq
Single Cell
Online Access:http://www.sciencedirect.com/science/article/pii/S2666166721004226

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http://www.sciencedirect.com/science/article/pii/S2666166721004226

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