Beneficial Effects of Isoflavones in the Kidney of Obese Rats Are Mediated by PPAR-Gamma Expression
Several studies have demonstrated an important association between altered lipid metabolism and the development of kidney injury because of a high-fat diet. Fructose is also closely associated with renal injury. We opted for a combination of fructose and saturated fats in a diet (DH) that is a model...
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MDPI AG
2020-06-01
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author | Edson de Andrade Pessoa Márcia Bastos Convento Bianca Castino Ala Moana Leme Andréia Silva de Oliveira Alef Aragão Sheila Marques Fernandes Adriana Carbonel Cassiane Dezoti Maria de Fátima Vattimo Nestor Schor Fernanda Teixeira Borges |
author_facet | Edson de Andrade Pessoa Márcia Bastos Convento Bianca Castino Ala Moana Leme Andréia Silva de Oliveira Alef Aragão Sheila Marques Fernandes Adriana Carbonel Cassiane Dezoti Maria de Fátima Vattimo Nestor Schor Fernanda Teixeira Borges |
author_sort | Edson de Andrade Pessoa |
collection | DOAJ |
description | Several studies have demonstrated an important association between altered lipid metabolism and the development of kidney injury because of a high-fat diet. Fructose is also closely associated with renal injury. We opted for a combination of fructose and saturated fats in a diet (DH) that is a model known to induce renal damage in order to evaluate whether soy isoflavones could have promising use in the treatment of renal alterations. After two months of ingestion, there was an expansion of visceral fat, which was associated with long-term metabolic disorders, such as sustained hyperglycemia, insulin resistance, polyuria, dyslipidemia, and hypertension. Additionally, we found a decrease in renal blood flow and an increase in renal vascular resistance. Biochemical markers of chronic kidney disease were detected; there was an infiltration of inflammatory cells with an elevated expression of proinflammatory cytokines (tumor necrosis factor-α, interleukin (IL)-6, and IL-1β), the activation of the renin–angiotensin system, and oxidative/nitrosative stress. Notably, in rats exposed to the DH diet for 120 days, the concomitant treatment with isoflavones after 60 days was able to revert metabolic parameters, renal alterations, and oxidative/nitrosative stress. The beneficial effects of isoflavones in the kidney of the obese rats were found to be mediated by expression of peroxisome proliferator-activated receptor gamma (PPAR-γ). |
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issn | 2072-6643 |
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last_indexed | 2024-03-10T19:28:00Z |
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spelling | doaj.art-b15d0d9b0ead42719554c28b93d4041c2023-11-20T02:26:27ZengMDPI AGNutrients2072-66432020-06-01126162410.3390/nu12061624Beneficial Effects of Isoflavones in the Kidney of Obese Rats Are Mediated by PPAR-Gamma ExpressionEdson de Andrade Pessoa0Márcia Bastos Convento1Bianca Castino2Ala Moana Leme3Andréia Silva de Oliveira4Alef Aragão5Sheila Marques Fernandes6Adriana Carbonel7Cassiane Dezoti8Maria de Fátima Vattimo9Nestor Schor10Fernanda Teixeira Borges11Nephrology Division, Department of Medicine, Universidade Federal de São Paulo, São Paulo SP 04023-900, BrazilNephrology Division, Department of Medicine, Universidade Federal de São Paulo, São Paulo SP 04023-900, BrazilInterdisciplinary Postgraduate Program in Health Sciences, Universidade Cruzeiro do Sul, São Paulo SP 01506-000, BrazilNephrology Division, Department of Medicine, Universidade Federal de São Paulo, São Paulo SP 04023-900, BrazilNephrology Division, Department of Medicine, Universidade Federal de São Paulo, São Paulo SP 04023-900, BrazilInterdisciplinary Postgraduate Program in Health Sciences, Universidade Cruzeiro do Sul, São Paulo SP 01506-000, BrazilExperimentation Laboratory in Animal Model, School of Nursing, Universidade de São Paulo, São Paulo SP 05403-000, BrazilHistology and Structural Biology Division, Department of Morphology and Genetics, Universidade Federal de São Paulo, São Paulo SP 04039-032, BrazilExperimentation Laboratory in Animal Model, School of Nursing, Universidade de São Paulo, São Paulo SP 05403-000, BrazilDepartment Medical-Surgical Nursing, School of Nursing, Universidade de São Paulo, São Paulo SP 05403-000, BrazilNephrology Division, Department of Medicine, Universidade Federal de São Paulo, São Paulo SP 04023-900, BrazilNephrology Division, Department of Medicine, Universidade Federal de São Paulo, São Paulo SP 04023-900, BrazilSeveral studies have demonstrated an important association between altered lipid metabolism and the development of kidney injury because of a high-fat diet. Fructose is also closely associated with renal injury. We opted for a combination of fructose and saturated fats in a diet (DH) that is a model known to induce renal damage in order to evaluate whether soy isoflavones could have promising use in the treatment of renal alterations. After two months of ingestion, there was an expansion of visceral fat, which was associated with long-term metabolic disorders, such as sustained hyperglycemia, insulin resistance, polyuria, dyslipidemia, and hypertension. Additionally, we found a decrease in renal blood flow and an increase in renal vascular resistance. Biochemical markers of chronic kidney disease were detected; there was an infiltration of inflammatory cells with an elevated expression of proinflammatory cytokines (tumor necrosis factor-α, interleukin (IL)-6, and IL-1β), the activation of the renin–angiotensin system, and oxidative/nitrosative stress. Notably, in rats exposed to the DH diet for 120 days, the concomitant treatment with isoflavones after 60 days was able to revert metabolic parameters, renal alterations, and oxidative/nitrosative stress. The beneficial effects of isoflavones in the kidney of the obese rats were found to be mediated by expression of peroxisome proliferator-activated receptor gamma (PPAR-γ).https://www.mdpi.com/2072-6643/12/6/1624high-fat/high-fructose dietnephropathyisoflavonesPPAR-γangiotensin IIoxidative stress |
spellingShingle | Edson de Andrade Pessoa Márcia Bastos Convento Bianca Castino Ala Moana Leme Andréia Silva de Oliveira Alef Aragão Sheila Marques Fernandes Adriana Carbonel Cassiane Dezoti Maria de Fátima Vattimo Nestor Schor Fernanda Teixeira Borges Beneficial Effects of Isoflavones in the Kidney of Obese Rats Are Mediated by PPAR-Gamma Expression Nutrients high-fat/high-fructose diet nephropathy isoflavones PPAR-γ angiotensin II oxidative stress |
title | Beneficial Effects of Isoflavones in the Kidney of Obese Rats Are Mediated by PPAR-Gamma Expression |
title_full | Beneficial Effects of Isoflavones in the Kidney of Obese Rats Are Mediated by PPAR-Gamma Expression |
title_fullStr | Beneficial Effects of Isoflavones in the Kidney of Obese Rats Are Mediated by PPAR-Gamma Expression |
title_full_unstemmed | Beneficial Effects of Isoflavones in the Kidney of Obese Rats Are Mediated by PPAR-Gamma Expression |
title_short | Beneficial Effects of Isoflavones in the Kidney of Obese Rats Are Mediated by PPAR-Gamma Expression |
title_sort | beneficial effects of isoflavones in the kidney of obese rats are mediated by ppar gamma expression |
topic | high-fat/high-fructose diet nephropathy isoflavones PPAR-γ angiotensin II oxidative stress |
url | https://www.mdpi.com/2072-6643/12/6/1624 |
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