Beneficial Effects of Isoflavones in the Kidney of Obese Rats Are Mediated by PPAR-Gamma Expression

Several studies have demonstrated an important association between altered lipid metabolism and the development of kidney injury because of a high-fat diet. Fructose is also closely associated with renal injury. We opted for a combination of fructose and saturated fats in a diet (DH) that is a model...

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Main Authors: Edson de Andrade Pessoa, Márcia Bastos Convento, Bianca Castino, Ala Moana Leme, Andréia Silva de Oliveira, Alef Aragão, Sheila Marques Fernandes, Adriana Carbonel, Cassiane Dezoti, Maria de Fátima Vattimo, Nestor Schor, Fernanda Teixeira Borges
Format: Article
Language:English
Published: MDPI AG 2020-06-01
Series:Nutrients
Subjects:
Online Access:https://www.mdpi.com/2072-6643/12/6/1624
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author Edson de Andrade Pessoa
Márcia Bastos Convento
Bianca Castino
Ala Moana Leme
Andréia Silva de Oliveira
Alef Aragão
Sheila Marques Fernandes
Adriana Carbonel
Cassiane Dezoti
Maria de Fátima Vattimo
Nestor Schor
Fernanda Teixeira Borges
author_facet Edson de Andrade Pessoa
Márcia Bastos Convento
Bianca Castino
Ala Moana Leme
Andréia Silva de Oliveira
Alef Aragão
Sheila Marques Fernandes
Adriana Carbonel
Cassiane Dezoti
Maria de Fátima Vattimo
Nestor Schor
Fernanda Teixeira Borges
author_sort Edson de Andrade Pessoa
collection DOAJ
description Several studies have demonstrated an important association between altered lipid metabolism and the development of kidney injury because of a high-fat diet. Fructose is also closely associated with renal injury. We opted for a combination of fructose and saturated fats in a diet (DH) that is a model known to induce renal damage in order to evaluate whether soy isoflavones could have promising use in the treatment of renal alterations. After two months of ingestion, there was an expansion of visceral fat, which was associated with long-term metabolic disorders, such as sustained hyperglycemia, insulin resistance, polyuria, dyslipidemia, and hypertension. Additionally, we found a decrease in renal blood flow and an increase in renal vascular resistance. Biochemical markers of chronic kidney disease were detected; there was an infiltration of inflammatory cells with an elevated expression of proinflammatory cytokines (tumor necrosis factor-α, interleukin (IL)-6, and IL-1β), the activation of the renin–angiotensin system, and oxidative/nitrosative stress. Notably, in rats exposed to the DH diet for 120 days, the concomitant treatment with isoflavones after 60 days was able to revert metabolic parameters, renal alterations, and oxidative/nitrosative stress. The beneficial effects of isoflavones in the kidney of the obese rats were found to be mediated by expression of peroxisome proliferator-activated receptor gamma (PPAR-γ).
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spelling doaj.art-b15d0d9b0ead42719554c28b93d4041c2023-11-20T02:26:27ZengMDPI AGNutrients2072-66432020-06-01126162410.3390/nu12061624Beneficial Effects of Isoflavones in the Kidney of Obese Rats Are Mediated by PPAR-Gamma ExpressionEdson de Andrade Pessoa0Márcia Bastos Convento1Bianca Castino2Ala Moana Leme3Andréia Silva de Oliveira4Alef Aragão5Sheila Marques Fernandes6Adriana Carbonel7Cassiane Dezoti8Maria de Fátima Vattimo9Nestor Schor10Fernanda Teixeira Borges11Nephrology Division, Department of Medicine, Universidade Federal de São Paulo, São Paulo SP 04023-900, BrazilNephrology Division, Department of Medicine, Universidade Federal de São Paulo, São Paulo SP 04023-900, BrazilInterdisciplinary Postgraduate Program in Health Sciences, Universidade Cruzeiro do Sul, São Paulo SP 01506-000, BrazilNephrology Division, Department of Medicine, Universidade Federal de São Paulo, São Paulo SP 04023-900, BrazilNephrology Division, Department of Medicine, Universidade Federal de São Paulo, São Paulo SP 04023-900, BrazilInterdisciplinary Postgraduate Program in Health Sciences, Universidade Cruzeiro do Sul, São Paulo SP 01506-000, BrazilExperimentation Laboratory in Animal Model, School of Nursing, Universidade de São Paulo, São Paulo SP 05403-000, BrazilHistology and Structural Biology Division, Department of Morphology and Genetics, Universidade Federal de São Paulo, São Paulo SP 04039-032, BrazilExperimentation Laboratory in Animal Model, School of Nursing, Universidade de São Paulo, São Paulo SP 05403-000, BrazilDepartment Medical-Surgical Nursing, School of Nursing, Universidade de São Paulo, São Paulo SP 05403-000, BrazilNephrology Division, Department of Medicine, Universidade Federal de São Paulo, São Paulo SP 04023-900, BrazilNephrology Division, Department of Medicine, Universidade Federal de São Paulo, São Paulo SP 04023-900, BrazilSeveral studies have demonstrated an important association between altered lipid metabolism and the development of kidney injury because of a high-fat diet. Fructose is also closely associated with renal injury. We opted for a combination of fructose and saturated fats in a diet (DH) that is a model known to induce renal damage in order to evaluate whether soy isoflavones could have promising use in the treatment of renal alterations. After two months of ingestion, there was an expansion of visceral fat, which was associated with long-term metabolic disorders, such as sustained hyperglycemia, insulin resistance, polyuria, dyslipidemia, and hypertension. Additionally, we found a decrease in renal blood flow and an increase in renal vascular resistance. Biochemical markers of chronic kidney disease were detected; there was an infiltration of inflammatory cells with an elevated expression of proinflammatory cytokines (tumor necrosis factor-α, interleukin (IL)-6, and IL-1β), the activation of the renin–angiotensin system, and oxidative/nitrosative stress. Notably, in rats exposed to the DH diet for 120 days, the concomitant treatment with isoflavones after 60 days was able to revert metabolic parameters, renal alterations, and oxidative/nitrosative stress. The beneficial effects of isoflavones in the kidney of the obese rats were found to be mediated by expression of peroxisome proliferator-activated receptor gamma (PPAR-γ).https://www.mdpi.com/2072-6643/12/6/1624high-fat/high-fructose dietnephropathyisoflavonesPPAR-γangiotensin IIoxidative stress
spellingShingle Edson de Andrade Pessoa
Márcia Bastos Convento
Bianca Castino
Ala Moana Leme
Andréia Silva de Oliveira
Alef Aragão
Sheila Marques Fernandes
Adriana Carbonel
Cassiane Dezoti
Maria de Fátima Vattimo
Nestor Schor
Fernanda Teixeira Borges
Beneficial Effects of Isoflavones in the Kidney of Obese Rats Are Mediated by PPAR-Gamma Expression
Nutrients
high-fat/high-fructose diet
nephropathy
isoflavones
PPAR-γ
angiotensin II
oxidative stress
title Beneficial Effects of Isoflavones in the Kidney of Obese Rats Are Mediated by PPAR-Gamma Expression
title_full Beneficial Effects of Isoflavones in the Kidney of Obese Rats Are Mediated by PPAR-Gamma Expression
title_fullStr Beneficial Effects of Isoflavones in the Kidney of Obese Rats Are Mediated by PPAR-Gamma Expression
title_full_unstemmed Beneficial Effects of Isoflavones in the Kidney of Obese Rats Are Mediated by PPAR-Gamma Expression
title_short Beneficial Effects of Isoflavones in the Kidney of Obese Rats Are Mediated by PPAR-Gamma Expression
title_sort beneficial effects of isoflavones in the kidney of obese rats are mediated by ppar gamma expression
topic high-fat/high-fructose diet
nephropathy
isoflavones
PPAR-γ
angiotensin II
oxidative stress
url https://www.mdpi.com/2072-6643/12/6/1624
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