Genetic Features of HIV-1 Integrase Sub-Subtype A6 Predominant in Russia and Predicted Susceptibility to INSTIs
The increasing use of the integrase strand transfer inhibitor (INSTI) class for the treatment of HIV-infection has pointed to the importance of analyzing the features of HIV-1 subtypes for an improved understanding of viral genetic variability in the occurrence of drug resistance (DR). In this study...
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MDPI AG
2020-07-01
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| Series: | Viruses |
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| Online Access: | https://www.mdpi.com/1999-4915/12/8/838 |
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| author | Alina Kirichenko Ilya Lapovok Pavel Baryshev David A. M. C. van de Vijver Jeroen J. A. van Kampen Charles A. B. Boucher Dimitrios Paraskevis Dmitry Kireev |
| author_facet | Alina Kirichenko Ilya Lapovok Pavel Baryshev David A. M. C. van de Vijver Jeroen J. A. van Kampen Charles A. B. Boucher Dimitrios Paraskevis Dmitry Kireev |
| author_sort | Alina Kirichenko |
| collection | DOAJ |
| description | The increasing use of the integrase strand transfer inhibitor (INSTI) class for the treatment of HIV-infection has pointed to the importance of analyzing the features of HIV-1 subtypes for an improved understanding of viral genetic variability in the occurrence of drug resistance (DR). In this study, we have described the prevalence of INSTI DR in a Russian cohort and the genetic features of HIV-1 integrase sub-subtype A6. We included 408 HIV infected patients who were not exposed to INSTI. Drug resistance mutations (DRMs) were detected among 1.3% of ART-naïve patients and among 2.7% of INSTI-naïve patients. The prevalence of 12 polymorphic mutations was significantly different between sub-subtypes A6 and A1. Analysis of the genetic barriers determined two positions in which subtype A (A1 and A6) showed a higher genetic barrier (G140C and V151I) compared with subtype B, and one position in which subtypes A1 and B displayed a higher genetic barrier (L74M and L74I) than sub-subtype A6. Additionally, we confirmed that the L74I mutation was selected at the early stage of the epidemic and subsequently spread as a founder effect in Russia. Our data have added to the overall understanding of the genetic features of sub-subtype A6 in the context of drug resistance. |
| first_indexed | 2024-03-10T18:03:27Z |
| format | Article |
| id | doaj.art-b16e5b5e1a8946f5ab8dc3bb0a5ecf8c |
| institution | Directory Open Access Journal |
| issn | 1999-4915 |
| language | English |
| last_indexed | 2024-03-10T18:03:27Z |
| publishDate | 2020-07-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Viruses |
| spelling | doaj.art-b16e5b5e1a8946f5ab8dc3bb0a5ecf8c2023-11-20T08:40:36ZengMDPI AGViruses1999-49152020-07-0112883810.3390/v12080838Genetic Features of HIV-1 Integrase Sub-Subtype A6 Predominant in Russia and Predicted Susceptibility to INSTIsAlina Kirichenko0Ilya Lapovok1Pavel Baryshev2David A. M. C. van de Vijver3Jeroen J. A. van Kampen4Charles A. B. Boucher5Dimitrios Paraskevis6Dmitry Kireev7Central Research Institute of Epidemiology, 111123 Moscow, RussiaCentral Research Institute of Epidemiology, 111123 Moscow, RussiaCentral Research Institute of Epidemiology, 111123 Moscow, RussiaViroscience Department, Erasmus Medical Centre, 3015 CE Rotterdam, The NetherlandsViroscience Department, Erasmus Medical Centre, 3015 CE Rotterdam, The NetherlandsViroscience Department, Erasmus Medical Centre, 3015 CE Rotterdam, The NetherlandsDepartment of Hygiene, Epidemiology and Medical Statistics, Medical School, National and Kapodistrian University of Athens, 11527 Goudi, Athens, GreeceCentral Research Institute of Epidemiology, 111123 Moscow, RussiaThe increasing use of the integrase strand transfer inhibitor (INSTI) class for the treatment of HIV-infection has pointed to the importance of analyzing the features of HIV-1 subtypes for an improved understanding of viral genetic variability in the occurrence of drug resistance (DR). In this study, we have described the prevalence of INSTI DR in a Russian cohort and the genetic features of HIV-1 integrase sub-subtype A6. We included 408 HIV infected patients who were not exposed to INSTI. Drug resistance mutations (DRMs) were detected among 1.3% of ART-naïve patients and among 2.7% of INSTI-naïve patients. The prevalence of 12 polymorphic mutations was significantly different between sub-subtypes A6 and A1. Analysis of the genetic barriers determined two positions in which subtype A (A1 and A6) showed a higher genetic barrier (G140C and V151I) compared with subtype B, and one position in which subtypes A1 and B displayed a higher genetic barrier (L74M and L74I) than sub-subtype A6. Additionally, we confirmed that the L74I mutation was selected at the early stage of the epidemic and subsequently spread as a founder effect in Russia. Our data have added to the overall understanding of the genetic features of sub-subtype A6 in the context of drug resistance.https://www.mdpi.com/1999-4915/12/8/838HIV-1A6integraseINSTIL74Ipolymorphisms |
| spellingShingle | Alina Kirichenko Ilya Lapovok Pavel Baryshev David A. M. C. van de Vijver Jeroen J. A. van Kampen Charles A. B. Boucher Dimitrios Paraskevis Dmitry Kireev Genetic Features of HIV-1 Integrase Sub-Subtype A6 Predominant in Russia and Predicted Susceptibility to INSTIs Viruses HIV-1 A6 integrase INSTI L74I polymorphisms |
| title | Genetic Features of HIV-1 Integrase Sub-Subtype A6 Predominant in Russia and Predicted Susceptibility to INSTIs |
| title_full | Genetic Features of HIV-1 Integrase Sub-Subtype A6 Predominant in Russia and Predicted Susceptibility to INSTIs |
| title_fullStr | Genetic Features of HIV-1 Integrase Sub-Subtype A6 Predominant in Russia and Predicted Susceptibility to INSTIs |
| title_full_unstemmed | Genetic Features of HIV-1 Integrase Sub-Subtype A6 Predominant in Russia and Predicted Susceptibility to INSTIs |
| title_short | Genetic Features of HIV-1 Integrase Sub-Subtype A6 Predominant in Russia and Predicted Susceptibility to INSTIs |
| title_sort | genetic features of hiv 1 integrase sub subtype a6 predominant in russia and predicted susceptibility to instis |
| topic | HIV-1 A6 integrase INSTI L74I polymorphisms |
| url | https://www.mdpi.com/1999-4915/12/8/838 |
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