Determination of internal controls for quantitative gene expression of Spodoptera litura under microbial pesticide stress

Abstract Quantitative real-time polymerase chain reaction (qRT-PCR) has become a commonly used method for the quantification of gene expression. However, accurate qRT-PCR analysis requires a valid internal reference for data normalization. To determine the valid reference characterized with low expr...

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Bibliographic Details
Main Authors: Shuang Wu, Yunmi Luo, Zhihong Zeng, Ying Yu, Shicai Zhang, Yan Hu, Lei Chen
Format: Article
Language:English
Published: Nature Portfolio 2024-03-01
Series:Scientific Reports
Online Access:https://doi.org/10.1038/s41598-024-56724-9