Generation of liver mesenchyme and ductal cell organoid co-culture using cell self-aggregation and droplet microfluidics

Summary: Within the peri-portal region of the adult liver, portal fibroblasts exist in close proximity to epithelial ductal/cholangiocyte cells. However, the cellular interactions between them are poorly understood. Here, we provide two co-culture techniques to incorporate liver portal mesenchyme in...

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Bibliographic Details
Main Authors: Anna M. Dowbaj, Timo N. Kohler, Lucía Cordero-Espinoza, Florian Hollfelder, Meritxell Huch
Format: Article
Language:English
Published: Elsevier 2023-06-01
Series:STAR Protocols
Subjects:
Online Access:http://www.sciencedirect.com/science/article/pii/S2666166723003003