A comprehensive survey of C. elegans argonaute proteins reveals organism-wide gene regulatory networks and functions
Argonaute (AGO) proteins associate with small RNAs to direct their effector function on complementary transcripts. The nematode Caenorhabditis elegans contains an expanded family of 19 functional AGO proteins, many of which have not been fully characterized. In this work, we systematically analyzed...
| Main Authors: | , , , , , , , , , |
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| Format: | Article |
| Language: | English |
| Published: |
eLife Sciences Publications Ltd
2023-02-01
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| Series: | eLife |
| Subjects: | |
| Online Access: | https://elifesciences.org/articles/83853 |
| _version_ | 1827967955654148096 |
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| author | Uri Seroussi Andrew Lugowski Lina Wadi Robert X Lao Alexandra R Willis Winnie Zhao Adam E Sundby Amanda G Charlesworth Aaron W Reinke Julie M Claycomb |
| author_facet | Uri Seroussi Andrew Lugowski Lina Wadi Robert X Lao Alexandra R Willis Winnie Zhao Adam E Sundby Amanda G Charlesworth Aaron W Reinke Julie M Claycomb |
| author_sort | Uri Seroussi |
| collection | DOAJ |
| description | Argonaute (AGO) proteins associate with small RNAs to direct their effector function on complementary transcripts. The nematode Caenorhabditis elegans contains an expanded family of 19 functional AGO proteins, many of which have not been fully characterized. In this work, we systematically analyzed every C. elegans AGO using CRISPR-Cas9 genome editing to introduce GFP::3xFLAG tags. We have characterized the expression patterns of each AGO throughout development, identified small RNA binding complements, and determined the effects of ago loss on small RNA populations and developmental phenotypes. Our analysis indicates stratification of subsets of AGOs into distinct regulatory modules, and integration of our data led us to uncover novel stress-induced fertility and pathogen response phenotypes due to ago loss. |
| first_indexed | 2024-04-09T18:13:46Z |
| format | Article |
| id | doaj.art-b1a76c696e384319b916740e4f28dd9a |
| institution | Directory Open Access Journal |
| issn | 2050-084X |
| language | English |
| last_indexed | 2024-04-09T18:13:46Z |
| publishDate | 2023-02-01 |
| publisher | eLife Sciences Publications Ltd |
| record_format | Article |
| series | eLife |
| spelling | doaj.art-b1a76c696e384319b916740e4f28dd9a2023-04-13T11:19:42ZengeLife Sciences Publications LtdeLife2050-084X2023-02-011210.7554/eLife.83853A comprehensive survey of C. elegans argonaute proteins reveals organism-wide gene regulatory networks and functionsUri Seroussi0Andrew Lugowski1Lina Wadi2Robert X Lao3Alexandra R Willis4Winnie Zhao5Adam E Sundby6Amanda G Charlesworth7Aaron W Reinke8https://orcid.org/0000-0001-7612-5342Julie M Claycomb9https://orcid.org/0000-0003-3132-5206Department of Molecular Genetics, University of Toronto, Toronto, CanadaDepartment of Molecular Genetics, University of Toronto, Toronto, CanadaDepartment of Molecular Genetics, University of Toronto, Toronto, CanadaDepartment of Molecular Genetics, University of Toronto, Toronto, CanadaDepartment of Molecular Genetics, University of Toronto, Toronto, CanadaDepartment of Molecular Genetics, University of Toronto, Toronto, CanadaDepartment of Molecular Genetics, University of Toronto, Toronto, CanadaDepartment of Molecular Genetics, University of Toronto, Toronto, CanadaDepartment of Molecular Genetics, University of Toronto, Toronto, CanadaDepartment of Molecular Genetics, University of Toronto, Toronto, CanadaArgonaute (AGO) proteins associate with small RNAs to direct their effector function on complementary transcripts. The nematode Caenorhabditis elegans contains an expanded family of 19 functional AGO proteins, many of which have not been fully characterized. In this work, we systematically analyzed every C. elegans AGO using CRISPR-Cas9 genome editing to introduce GFP::3xFLAG tags. We have characterized the expression patterns of each AGO throughout development, identified small RNA binding complements, and determined the effects of ago loss on small RNA populations and developmental phenotypes. Our analysis indicates stratification of subsets of AGOs into distinct regulatory modules, and integration of our data led us to uncover novel stress-induced fertility and pathogen response phenotypes due to ago loss.https://elifesciences.org/articles/83853ArgonautemiRNApiRNA22G-RNA26G-RNAgene regulation |
| spellingShingle | Uri Seroussi Andrew Lugowski Lina Wadi Robert X Lao Alexandra R Willis Winnie Zhao Adam E Sundby Amanda G Charlesworth Aaron W Reinke Julie M Claycomb A comprehensive survey of C. elegans argonaute proteins reveals organism-wide gene regulatory networks and functions eLife Argonaute miRNA piRNA 22G-RNA 26G-RNA gene regulation |
| title | A comprehensive survey of C. elegans argonaute proteins reveals organism-wide gene regulatory networks and functions |
| title_full | A comprehensive survey of C. elegans argonaute proteins reveals organism-wide gene regulatory networks and functions |
| title_fullStr | A comprehensive survey of C. elegans argonaute proteins reveals organism-wide gene regulatory networks and functions |
| title_full_unstemmed | A comprehensive survey of C. elegans argonaute proteins reveals organism-wide gene regulatory networks and functions |
| title_short | A comprehensive survey of C. elegans argonaute proteins reveals organism-wide gene regulatory networks and functions |
| title_sort | comprehensive survey of c elegans argonaute proteins reveals organism wide gene regulatory networks and functions |
| topic | Argonaute miRNA piRNA 22G-RNA 26G-RNA gene regulation |
| url | https://elifesciences.org/articles/83853 |
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