Comparison of four DNA extraction methods for the detection of Mycobacterium leprae from Ziehl–Neelsen-stained microscopic slides

Comparison of four DNA extraction methods for the detection of Mycobacterium leprae from Ziehl–Neelsen-stained microscopic slides

Objective/background: The diagnosis of leprosy has been a challenge due to the low sensibility of the conventional methods and the impossibility of culturing the causative organism. In this study, four methods for Mycobacterium leprae nucleic-acid extraction from Ziehl–Neelsen-stained slides (ZNS sl...

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Bibliographic Details
Main Authors: Jenny Laura Ruiz-Fuentes, Alexis Díaz, Anayma Elena Entenza, Yahima Frión, Odelaisy Suárez, Pedro Torres, Yaxsier de Armas, Lucrecia Acosta
Format: Article
Language:English
Published: Wolters Kluwer Medknow Publications 2015-01-01
Series:International Journal of Mycobacteriology
Subjects:
DNA extraction methods
Leprosy
Molecular diagnosis
Mycobacterium leprae
Ziehl–Neelsen-stained microscopic slides
Online Access:http://www.ijmyco.org/article.asp?issn=2212-5531;year=2015;volume=4;issue=4;spage=284;epage=289;aulast=Ruiz-Fuentes

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http://www.ijmyco.org/article.asp?issn=2212-5531;year=2015;volume=4;issue=4;spage=284;epage=289;aulast=Ruiz-Fuentes

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