Single-cell analysis of the cellular landscape of vulvar melanoma provides new insight for immunotherapy administration
Abstract Background Vulvar and vaginal melanoma (VuM & VaM) is a rare gynecologic malignancy with high mortality but low effectiveness to checkpoint immunotherapy compared to cutaneous melanoma. This article aims to elucidate the role of the disordered immune microenvironment in cancer progressi...
| Main Authors: | , , , , , , |
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| Format: | Article |
| Language: | English |
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BMC
2024-01-01
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| Series: | BMC Cancer |
| Subjects: | |
| Online Access: | https://doi.org/10.1186/s12885-024-11839-0 |
| _version_ | 1797349876928872448 |
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| author | Xinqi Wang Jiahui Li Yifei Li Mingyi Lv Xue Dong Zhenxin Fan Tao Guo |
| author_facet | Xinqi Wang Jiahui Li Yifei Li Mingyi Lv Xue Dong Zhenxin Fan Tao Guo |
| author_sort | Xinqi Wang |
| collection | DOAJ |
| description | Abstract Background Vulvar and vaginal melanoma (VuM & VaM) is a rare gynecologic malignancy with high mortality but low effectiveness to checkpoint immunotherapy compared to cutaneous melanoma. This article aims to elucidate the role of the disordered immune microenvironment in cancer progression in VuM. Methods At first, this article applied single-cell RNA sequencing (scRNA-seq) to the VuM obtained from a 68-year-old female patient, and constructed a single-cell atlas of VuM consist of 12,243 single cells. Then this article explores the genomic complexity and core signal channel in VuM microenvironment. Results This article provides new insights about the pathogenesis of VuM based on single-cell resolution data. It was found that the activation of CD8+ T cell contributed to induce tumor angiogenesis and immune escape, and the activation of the antigen-presenting molecular function participated in melanoma metastasis. Conclusion This article provided new insights into underlining VuM molecular regulation and potential signaling involved in immunotherapy, which would benefit the clinical practice and administration. |
| first_indexed | 2024-03-08T12:36:43Z |
| format | Article |
| id | doaj.art-b1bc43dbd61d4f10909f6caedae1c41e |
| institution | Directory Open Access Journal |
| issn | 1471-2407 |
| language | English |
| last_indexed | 2024-03-08T12:36:43Z |
| publishDate | 2024-01-01 |
| publisher | BMC |
| record_format | Article |
| series | BMC Cancer |
| spelling | doaj.art-b1bc43dbd61d4f10909f6caedae1c41e2024-01-21T12:24:46ZengBMCBMC Cancer1471-24072024-01-0124111210.1186/s12885-024-11839-0Single-cell analysis of the cellular landscape of vulvar melanoma provides new insight for immunotherapy administrationXinqi Wang0Jiahui Li1Yifei Li2Mingyi Lv3Xue Dong4Zhenxin Fan5Tao Guo6Key Laboratory of Bioresources and Eco-environment (Ministry of Education), College of Life Sciences, Sichuan UniversityKey Laboratory of Bioresources and Eco-environment (Ministry of Education), College of Life Sciences, Sichuan UniversityKey Laboratory of Birth Defects and Related Diseases of Women and Children of MOE, Department of Pediatrics, West China Second University Hospital, Sichuan UniversityKey Laboratory of Bioresources and Eco-environment (Ministry of Education), College of Life Sciences, Sichuan UniversityAmbulatory surgery Department, West China Second Hospital, Sichuan UniversityKey Laboratory of Bioresources and Eco-environment (Ministry of Education), College of Life Sciences, Sichuan UniversityDepartment of Gynecology and Obstetrics, West China Second University Hospital, Sichuan UniversityAbstract Background Vulvar and vaginal melanoma (VuM & VaM) is a rare gynecologic malignancy with high mortality but low effectiveness to checkpoint immunotherapy compared to cutaneous melanoma. This article aims to elucidate the role of the disordered immune microenvironment in cancer progression in VuM. Methods At first, this article applied single-cell RNA sequencing (scRNA-seq) to the VuM obtained from a 68-year-old female patient, and constructed a single-cell atlas of VuM consist of 12,243 single cells. Then this article explores the genomic complexity and core signal channel in VuM microenvironment. Results This article provides new insights about the pathogenesis of VuM based on single-cell resolution data. It was found that the activation of CD8+ T cell contributed to induce tumor angiogenesis and immune escape, and the activation of the antigen-presenting molecular function participated in melanoma metastasis. Conclusion This article provided new insights into underlining VuM molecular regulation and potential signaling involved in immunotherapy, which would benefit the clinical practice and administration.https://doi.org/10.1186/s12885-024-11839-0Vulvar melanomascRNA-seqImmune microenvironmentImmune cells; Case report |
| spellingShingle | Xinqi Wang Jiahui Li Yifei Li Mingyi Lv Xue Dong Zhenxin Fan Tao Guo Single-cell analysis of the cellular landscape of vulvar melanoma provides new insight for immunotherapy administration BMC Cancer Vulvar melanoma scRNA-seq Immune microenvironment Immune cells; Case report |
| title | Single-cell analysis of the cellular landscape of vulvar melanoma provides new insight for immunotherapy administration |
| title_full | Single-cell analysis of the cellular landscape of vulvar melanoma provides new insight for immunotherapy administration |
| title_fullStr | Single-cell analysis of the cellular landscape of vulvar melanoma provides new insight for immunotherapy administration |
| title_full_unstemmed | Single-cell analysis of the cellular landscape of vulvar melanoma provides new insight for immunotherapy administration |
| title_short | Single-cell analysis of the cellular landscape of vulvar melanoma provides new insight for immunotherapy administration |
| title_sort | single cell analysis of the cellular landscape of vulvar melanoma provides new insight for immunotherapy administration |
| topic | Vulvar melanoma scRNA-seq Immune microenvironment Immune cells; Case report |
| url | https://doi.org/10.1186/s12885-024-11839-0 |
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