Fluorescent protein tagging as a tool to define the subcellular distribution of proteins in plants
Fluorescent protein (FP) tagging approaches are widely used to determine the subcellular location of plant proteins. Here we give a brief overview of FP approaches, highlight potential technical problems, and discuss what to consider when designing FP/protein fusion constructs and performing transfo...
Main Authors: | , , , |
---|---|
Format: | Article |
Language: | English |
Published: |
Frontiers Media S.A.
2013-06-01
|
Series: | Frontiers in Plant Science |
Subjects: | |
Online Access: | http://journal.frontiersin.org/Journal/10.3389/fpls.2013.00214/full |